Anti-TP53(R282W) Mouse Monoclonal Antibody
英文名称： Anti-TP53(R282W) Mouse Monoclonal Antibody
型号:null    抗体货号： 26076
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

TP53(R282W) mono [26076]
[100 µL]		  

Catalog Number:  26076
Gene Symbol:  TP53
Description:   Anti-TP53(R282W) Mouse Monoclonal Antibody
Background:   p53 protein acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are found in many types of cancers.
Immunogen:   Recombinant p53(R282W) protein, human origin.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:1000-1:5000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG1
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-TP53(R282W) antibody recognizes TP53(R282W) of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant TP53(R282W) and wild-type p53 proteins. Purified His-tagged TP53(R282W) protein (lane 2, Cat.#10204) and wild type p53 protein (lane 1, Cat.#10202) were blotted with anti-TP53 (R282W) mouse monoclonal antibody (Cat. # 26076).
Immunofluorescence:  
Immunofluorescence of cells expressing TP53 proteins with anti-TP53 (R282W) antibody. HEK293T cells were transfected with pCDNA3-GFP-TP53 (WT) plasmid (left column) or pCDNA3-GFP-TP53 (R282W) plasmid (right column), then fixed and stained with anti- TP53 (R282W) monoclonal antibody (Cat. # 26076).