Anti-BRAF (D594G) Mouse Monoclonal Antibody
英文名称：  Anti-BRAF (D594G) Mouse Monoclonal Antibody
型号:null    抗体货号： 26200
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

BRAF (D594G) [26200]
[100ul]		  

Catalog Number:  26200
Gene Symbol:  BRAF, BRAF1, RAFB1
Description:   Anti-BRAF (D594G) Mouse Monoclonal Antibody
Background:   BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
Immunogen:   A synthetic peptide from the internal region of BRAF which includes the mutation of D594G, human origin.
Applications:   ELISA, WB
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes D594G mutant, but not wild-type BRAF of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  

 

Western blot analysis of recombinant BRAF (D594G) and wildtype proteins. Purified His-tagged BRAF (D594G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-BRAF (D594G) monoclonal antibody (Cat. #26200).