Anti-RHEB (Y35N) Mouse Monoclonal Antibody
英文名称： Anti-RHEB (Y35N) Mouse Monoclonal Antibody
型号:null    抗体货号： 26338
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

RHEB (Y35N) [26338]
[100ul]		  

Catalog Number:  26338
Gene Symbol:  RHEB
Description:   Anti-RHEB (Y35N) Mouse Monoclonal Antibody
Background:   RheB (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin's GAP activity.
Immunogen:   A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes Y35N mutant, but not wild-type RHEB of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant RHEB (Y35N) and wildtype proteins. Purified His-tagged RHEB (Y35N) protein (lane2) and corresponding wild-type protein (lane1) were blotted with anti-RHEB (Y35N) monoclonal antibody (Cat. #26338).
Immunofluorescence:  
Immunofluorescence of cells expressing RHEB proteins with anti-RHEB (Y35N) antibody. HEK293T cells were transfected with pCDNA3-GFP-RHEB (WT) plasmid (left column) or pCDNA3-GFP-RHEB (Y35N) plasmid (right column), then fixed and stained with anti-RHEB (Y35N) monoclonal antibody (Cat. #26338).

 

Publications:
1.  Inducible Nitric Oxide Synthase Drives mTOR Pathway Activation and Proliferation of Human Melanoma by Reversible Nitrosylation of TSC2
    Cancer Res. 2014 Feb 15;74(4):1067-78
2.  SynGAP Regulates Protein Synthesis and Homeostatic Synaptic Plasticity in Developing Cortical Networks
    PLoS One. 2013 Dec 31;8(12):e83941