Anti-TP53(R175H) Mouse Monoclonal Antibody
英文名称： Anti-TP53(R175H) Mouse Monoclonal Antibody
型号:null    抗体货号： 26072
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

TP53 (R175H) mono [26072]
[100 µL]		  

Catalog Number:  26072
Gene Symbol:  TP53(R175H)
Description:    Anti-TP53(R175H) Mouse Monoclonal Antibody
Background:   TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on.
Immunogen:   A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:1000-1:1000
IHC: 1:50-1:100
Concentration:    1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-TP53(R175H) antibody recognizes TP53(R175H) of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant TP53(R175H) and wildtype proteins. Purified His-tagged TP53(R175H) protein(Cat.#10207) and wildtype protein(Cat.#10202) were blotted with anti-TP53(R175H) monoclonal antibody(Cat.# 26072).
Immunofluorescence:  
Immunofluorescence of cells expressing TP53 proteins with anti-TP53 (R175H) antibody. HEK293T cells were transfected with pCDNA3-GFP-TP53 (WT) plasmid (left column) or pCDNA3-GFP-TP53 (R175H) plasmid (right column), then fixed and stained with anti- TP53 (R175H) monoclonal antibody (Cat. # 26072).