寨卡病毒快速检测卡
英文名称： ZIKA
型号:null    产品货号： 寨卡病毒快速检测卡
价格:请致电：010-57128832,18610462672
品牌: usa

 ZIKA病毒抗体IgG/IgM快速检测试剂（胶体金法）使用说明书

 
【产品名称】
通用名称：寨卡病毒IgG/IgM抗体快速诊断试剂盒（胶体金法）
【使用目的】
1.门诊病人的快速初查
2.疫区可疑人群的现场快速初查
3.健康人群体检
【试验原理】
寨卡病毒抗体快速诊断试剂盒（胶体金法）采用胶体金免疫层析技术，在玻璃纤维纸上预包被金标记摘卡重组抗原，在纤维膜上检测线和对照线处分别包被抗人IgGΥ链单克隆抗体、抗人IgMμ链单克隆抗体和兔抗摘卡抗体。检测阳性样本时，样本血清（浆）中寨卡抗体（IgG或IgM）与胶体金标记寨卡重组抗原(Au-Dengue-Ag)结合形成复合物，由于层析作用复合物沿纸条向前移动，经过检测线时与预包被的抗人IgGΥ链单克隆抗体或抗人IgMμ链单克隆抗体形成“Au-Zika-Ag-抗Zika抗体-抗人IgGΥ（或抗人IgMμ）-固相材料”夹心物而凝聚显色，游离金标抗原则在对照线处与兔抗寨卡抗体结合而富集显色。阴性标本则仅在对照线处显色。检查中把经过适当稀释的血清（浆）标本加到检测卡的加样处，20分钟内观察结果即可。
【试剂盒组成】
1. 寨卡病毒抗体检测试剂25人份/盒
2. 样品缓冲稀释液4ml
3.   说明书1份
【使用方法】
1．用加样器吸取血清或者血浆(浆)2ul，加在加样孔的上端（S1）处；
2．在加样孔（S）处滴加100ul（2滴）样品稀释液；
3．20分钟内观察并记录结果。
【对试验结果的解释】
阳性：1、IgG 阳性：在IgG检测线处和对照线位置出现两条紫红线。
2、IgM 阳性：在IgM检测线处和对照线位置出现两条紫红线。
3、IgG、IgM 同时阳性：在IgG检测线处、IgM检测线处和对照线位置同时出现三条紫红线。
阴性：只在对照线位置出现一条紫红线。
无效：在对照线位置没有出现紫红线。
【注意事项】
1. 检测必须符合传染病实验室管理规范和生物安全守则的规定。所有样品和废弃物都应按传染物处理；
2．操作应严格按说明书进行；
3．实验环境应保持一定湿度，避风，避免在过高温度下进行实验（最好在10～37℃）；
4．检测试剂从铝箔袋中取出后，应尽快进行实验，避免放置于空气中过长时间，试剂吸潮后将失效；
5．试剂盒可在室温下保存，谨防受潮，低温下保存的试剂盒应平衡至室温方可打开铝箔袋进行检测操作；
6．为了防止弱阳性标本的漏检，应在加样后20 分钟判读结果，在检测线处出现紫红线，即可判断为阳性结果，超过20 分钟后结果只作为参考。
【贮存】
4～30℃，密封干燥。
【有效期】18个月。
 

the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity).
Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).
Non-structural protein 1 is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome (By similarity).
Dengue virus envelope glycoprotein E,登革热病毒包膜糖蛋白E抗体Non-structural protein 2A may be involved viral RNA replication and capsid assembly (Potential).
Non-structural protein 2B is a required cofactor for the serine protease function of NS3 (By similarity).
Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity).
Non-structural protein 4A induces host endoplasmic reticulum membrane rearrangements leading to the formation of virus-induced membranous vesicles hosting the dsRNA and polymerase, functioning as a replication complex. NS4A might also regulate the ATPase activity of the NS3 helicase (By similarity).
Peptide 2k functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter (By similarity).
Non-structural protein 4B inhibits interferon (IFN)-induced host STAT1 phosphorylation and nuclear translocation, thereby preventing the establishment of cellular antiviral state by blocking the IFN-alpha/beta pathway (By similarity).
RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. Besides its role in genome replication, also prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) signaling pathway. Inhibits host TYK2 and STAT2 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway.

Subcellular Location:
Capsid protein C: Virion (Potential).
Peptide pr: Secreted.
Small envelope protein M: Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein.
Envelope protein E: Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein.
Non-structural protein 1: Secreted. Host endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side.
Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential).
Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential).
Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Note=Remains non-covalently associated to NS3 protease (By similarity).
Non-structural protein 4A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Located in RE-associated vesicles hosting the replication complex.
Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).
RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Host nucleus. Note=Located in RE-associated vesicles hosting the replication complex.

Post-translational modifications:
Specific enzymatic cleavages in vivo yield mature proteins. The nascent protein C contains a C-terminal hydrophobic domain that act as a signal sequence for translocation of prM into the lumen of the ER. Mature protein C is cleaved at a site upstream of this hydrophobic domain by NS3. prM is cleaved in post-Golgi vesicles by a host furin, releasing the mature small envelope protein M, and peptide pr. Non-structural protein 2A-alpha, a C-terminally truncated form of non-structural protein 2A, results from partial cleavage by NS3. Peptide 2K acts as a signal sequence and is removed from the N-terminus of NS4B by the host signal peptidase in the ER lumen. Signal cleavage at the 2K-4B site requires a prior NS3 protease-mediated cleavage at the 4A-2K site.
RNA-directed RNA polymerase NS5 is phosphorylated on serines residues. This phosphorylation may trigger NS5 nuclear localization.
Envelope protein E and non-structural protein 1 are N-glycosylated.

Dengue virus envelope glycoprotein E,登革热病毒包膜糖蛋白E抗体Similarity:
In the N-terminal section; belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0-1 NS5-type methyltransferase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 mRNA cap 0-1 NS5-type MT domain.
Contains 1 peptidase S7 domain.
Contains 1 RdRp catalytic domain.

Gene ID:
1494449

Database links:
Entrez Gene: 1494449 Dengue virus 2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

登革病毒(Dengue Virus，DEN)属黄病毒科(flavlviridae)又称汉坦病毒、黄病毒，是重要的虫媒病毒之一，是引起流行性出血热的主要原因。