anti-survivin Mouse Monoclonal Antibody
英文名称： anti-survivin Mouse Monoclonal Antibody
型号:null    抗体货号： 26131
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 
Description:   anti-survivin Mouse Monoclonal Antibody
Background:   Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin protein negatively regulate of programmed cell death by inhibiting caspase. Survivin protein is high expressed in most human tumours and fetal tissue, but is absent in differentiated cells. This makes survivin an ideal target for cancer therapy. Survivin is only expressed in the G2-M phase cells. It also plays a role in regulating mitosis.
Immunogen:   Purified survivin protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200
Concentration:   1mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes survivin of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant PTEN protein. Purified His-tagged Survivin protein was blotted with anti-survivin mouse monoclonal antibody (Cat. #26131).
Immunohistochemistry:   

 

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-survivin monoclonal antibody (Cat. #26131). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.