QuantiBRITE Reagents 荧光分子定量相关试剂 | Catalog Number | 340768 | | Brand | BD Quantibrite™ | | Name | CD64/CD45 | | Size | 50 tests | | Regulatory Status | RUO (GMP) | | Catalog Number | 340768 |
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| MD22 | PE | Mouse IgG1 | |
| 2D1 | PerCP | Mouse IgG1, k | |
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| CD64 recognizes the 72-kDa human FcgRI glycoprotein that can bind monomeric IgG.CD45 (Anti–HLe-1) recognizes human leucocyte antigens, Mr 180 to 220 kDa, that are members of the T200 family.The CD64 antigen is one of three Fc receptors for immunoglobulins, including human FcgRII (CD32 antigen) and human FcgRIII (CD16 antigen), that are present on the surface of leucocytes. While FcgRII and FcgRIII are low affinity receptors for immunoglobulin, FcgRI binds with high affinity. Structurally, the CD64 antigen possesses an extracellular region of 292 amino acids with three C2 set Ig-like domains, a 21-amino acid transmembrane region, and a charged cytoplasmic tail of 61 amino acids.The CD64 antigen is expressed on monocytes, macrophages, at very low levels on polymorpho-nuclear neutrophils (PMNs), and on a subpopulation of circulating dendritic cells. CD64 is the earliest granulomonocytic lineage marker on CD34+ hemopoietic progenitors. Carbocyanine conjugates, for example, phycoerythrin-Cy5 (PE-Cy5), bind to CD64. Soluble human FcgRI molecules have been found in human serum. Three genes have been characterized for FcgRI, each gene consisting of six exons, spanning 9.4 kilobases, and localized to chromosome 1.Binding of IgG to the Fc receptor mediates most of the leucocyte functions, including phagocytosis, endocytosis, cytolysis, cytokine release, and antibody-dependent cell cytotoxicity. CD64 binding to human FcgRI neutrophils has been shown to be an indicator of acute inflammation, as well as a potential tool for monitoring interferon-g (IFN-g) therapy. Quantitative CD64 expression on neutrophils is being studied as an indicator of infection or systemic acute inflammatory response. FcgRI is upregulated on PMNs, monocytes, and dendritic cells by IFN-g. Additionally, FcgRI is upregulated on PMNs by granulocyte-colony stimulating factor (G-CSF). FcgRI plays an important role in enhancing antigen presentation. Stable expression of FcgRI requires coexpression of the IgG-binding a-chain as an oligomeric complex with the FcR g-chain homodimer. |
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