美国Brand,null 产品货号： BTIDS001Item#, AdvCell® 组织/细胞分离消化液<br>英文名称： Tissue/Cell Isolation & Digestion Solution

AdvCell® 组织/细胞分离消化液
英文名称： Tissue/Cell Isolation & Digestion Solution

型号:null 产品货号： BTIDS001

价格:请致电：010-57128832,18610462672

品牌: 美国

idely recognised as a biomarker in relation to osteoporosis and osteopenia. It has also been shown to play a prominent role in immune response, cardiovascular disease and certain types of cancer. Vitamin D analysis, therefore, carries significance in several respects.

25-OH-vitamin D3 constitutes the principal diagnostic target and is the main metabolite of vitamin D in humans; it is the precursor of the physiologically most active compound 1,25-(OH)2-Vitamin D3. Vitamin D deficiency is treated with vitamin D3 or vitamin D2 supplementation. Vitamin D2 is easier to produce synthetically and, where prescribed, its monitoring is also necessary.

This reagent kit allows for the specific analysis of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma by tandem mass spectrometry. Manual sample preparation is minimised and limited to simple and effective protein precipitation. A subsequent online trap column concentrates the analytes and separates interfering substances. A six-port-valve connects the trap column to an HPLC column which provides further purification. The use of atmospheric pressure chemical ionisation (APCI) and an isotopically labelled internal standard assures precision and robustness and minimises ion suppression effects. The 3PLUS1® multilevel calibrators offer additional precision.

The upgrade to MassChrom® 25-OH-vitamin D3/D2 analysis allows for the separation of the main metabolites of vitamins D3 and D2, 25-Hydroxycholecalciferol and 25-Hydroxyergocalciferol, from their epimeric forms 3-epi-25-OH-vitamin D3 and 3-epi-25-OH-vitamin D2, hence allowing for separate quantification by LC MS/MS.

/TD>388 > 85C5DC-Carnitine-D6394 > 85C6-Carnitine316 > 85C6-Carnitine-D3319 > 85C8-Carnitine344 > 85C8-Carnitine-D3347 > 85C10-Carnitine372 > 85C10-Carnitine-D3375 > 85C12-Carnitine400 > 85C12-Carnitine-D3403 > 85C14-Carnitine428 > 85C14-Carnitine-D3431 > 85C16-Carnitine456 > 85C16-Carnitine-D3459 > 85C18-Carnitine484 > 85C18-Carnitine-D3487 > 85

Sample preparation (general)

Punch a 3 mm dried blood spot disk out of the filter card into a well of a microtiter plate (or a filter plate).
Add 200 µl of the reconstituted Internal Standard, seal with a protective sheet and agitate for 20 min at 600 rpm and room temperature.
Transfer the supernatant into a new microtiter plate. When using a filter plate draw through the supernatant into the well plate by centrifugation (4000 rpm, 2 minutes). Discard the filter plate.
Evaporate at 60 °C and 600 rpm to dryness.
Add 60 µl Derivatisation Reagent, seal microtiter plate with a protective sheet and incubate 15 min at 60 °C and 600 rpm.
Evaporate at 60 °C and 600 rpm to dryness.
Add 100 µl Reconstitution Buffer and agitate 1 min at 600 rpm.
Inject 10 µl into the LC-MS/MS system.

Sample preparation (succinylacetone)

Punch a 3 mm dried blood spot disk out of the filter card into a 96 well plate (or a filter plate).
Add 200 µl Internal Standard, seal with a protective sheet and agitate for 20 min at 600 rpm and room temperature.
Transfer the supernatant into a new 96 well plate. When using a filter plate draw through the supernatant into the 96 well plate by centrifugation (4000 rpm, 2 minutes).
Add 150 µl Internal Standard Succinylacetone to the remaining blood spot and agitate for 30 min at 60 °C.
Pool the supernatants and evaporate at 60 °C and 600 rpm to dryness.
Add 60 µl Derivatisation Reagent, seal microtiter plate with a protective sheet and incubate 15 min at 60 °C and 600 rpm.
Evaporate at 60 °C and 600 rpm to dryness.
Add 200 µl Reconstitution Buffer and agitate 1 min at 600 rpm.
Inject 10 µl into the LC-MS/MS system.

Order no. Product
55000 MassChrom^® Reagent kit for the determination of Amino Acids and Acylcarnitines from dried blood spot samples (LC-MS/MS) with regular 96 well plates.
For 960 analyses.
55000/F MassChrom^® Reagent kit for the determination of Amino Acids and Acylcarnitines from dried blood spot samples (LC-MS/MS) with 96 well filter plates.
For 960 analyses.
Separately available components
55001 Mobile Phase, 1000 ml
55002 Mobile Phase, 10 x 1000 ml
55004 Internal Standard (lyoph.), 4 x 50 ml
55044 Internal Standard for Succinylacetone, 3 x 50 ml
55004/T Internal Standard (lyoph.), 50 ml
55044/T Internal Standard for Succinylacetone, 50 ml
55005 Derivatisation Reagent, 30 ml
55006 Reconstitution Buffer, 100 ml
55007 Rinsing Solution, 1000 ml
55008 Extraction Buffer, 200 ml
55010 96-Well-Plates, 10 pcs.
55011 Protective sheets for 96 well plates, 10 pcs.
55057 96-Well-Filter-Plates, 5 pcs.
Succinylacetone Upgrade Set
55111 Succinylacetone Upgrade Set for 960 analyses, consisting of:
Internal Standard for Succinylacetone, 3 x 50 ml
Reconstitution Buffer, 100 ml
Startup accessories
55013 Pierceable adhesive seal for 96 well plates, 10 pcs.
55015 Restrictor Capillary, 1 pc.
55016 Adapter collar for centrifugation, 2 pcs.
55098 Tuning Mix for Succinylacetone, 1 ml
55099 Tuning Mix incl. all analytes, 2 ml
55033 PEEK prefilter, 5 µm, 5 pcs.
15010 PEEK prefilter housing, 1 pc.
Chromsystems quality control material (filter spot):
0191 MassCheck^® Amino Acids, Acylcarnitines Dried Blood Spot Control, Bi-Level (I+II), 2 x 3 Spots
0192 MassCheck^® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level I, 1 x 3 spots
0193 MassCheck^® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level II, 1 x 3 spots

Order no.	Product
55000	MassChrom^® Reagent kit for the determination of Amino Acids and Acylcarnitines from dried blood spot samples (LC-MS/MS) with regular 96 well plates. For 960 analyses.
55000/F	MassChrom^® Reagent kit for the determination of Amino Acids and Acylcarnitines from dried blood spot samples (LC-MS/MS) with 96 well filter plates. For 960 analyses.
	Separately available components
55001	Mobile Phase, 1000 ml
55002	Mobile Phase, 10 x 1000 ml
55004	Internal Standard (lyoph.), 4 x 50 ml
55044	Internal Standard for Succinylacetone, 3 x 50 ml
55004/T	Internal Standard (lyoph.), 50 ml
55044/T	Internal Standard for Succinylacetone, 50 ml
55005	Derivatisation Reagent, 30 ml
55006	Reconstitution Buffer, 100 ml
55007	Rinsing Solution, 1000 ml
55008	Extraction Buffer, 200 ml
55010	96-Well-Plates, 10 pcs.
55011	Protective sheets for 96 well plates, 10 pcs.
55057	96-Well-Filter-Plates, 5 pcs.
	Succinylacetone Upgrade Set
55111	Succinylacetone Upgrade Set for 960 analyses, consisting of:
	Internal Standard for Succinylacetone, 3 x 50 ml
	Reconstitution Buffer, 100 ml
	Startup accessories
55013	Pierceable adhesive seal for 96 well plates, 10 pcs.
55015	Restrictor Capillary, 1 pc.
55016	Adapter collar for centrifugation, 2 pcs.
55098	Tuning Mix for Succinylacetone, 1 ml
55099	Tuning Mix incl. all analytes, 2 ml
55033	PEEK prefilter, 5 µm, 5 pcs.
15010	PEEK prefilter housing, 1 pc.
	Chromsystems quality control material (filter spot):
0191	MassCheck^® Amino Acids, Acylcarnitines Dried Blood Spot Control, Bi-Level (I+II), 2 x 3 Spots
0192	MassCheck^® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level I, 1 x 3 spots
0193	MassCheck^® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level II, 1 x 3 spots

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型号

FC-20

培养基成分(1000ml)

巯基乙酸钠　－　1.5g
***酸氢二钠　－　1.1g
氯化钠　－　10.0g
氯化钙　－0.09g
琼脂　－　3.0g
pH 指示剂　－　0.012g

pH 8.4 ±0.2

用途

大便取样和运输

容器材料

聚丙烯

容器尺寸

¢10mmX80mm

培养基

Modified Carry-Blair
培养基1ml

包装个数

300支 (30支/纸盒)

保存方法

室温

有效期限

生产日期后1年

其他

被检者姓名标签