Background
The ZytoFast ® CMV-CISH System is desi-gned for the detection of cytomegalovirus (CMV) DNA in paraffin-embedded tissue sections or cell samples. CMV (a.k.a. human herpesvirus-5, HHV-5) is a member of the beta-herpesvirus group and may be found in 40-100% of people. CMV can be transmitted sexually as well as via breast milk, transplanted organs, and rarely from blood transfusions.
Following primary CMV infection in the normal host, the virus remains in a latent state and can be found in multiple body sites as it is, unlike other herpesviruses, not restricted to certain body areas. Among immunosuppressed patients, such as reci-pients of solid organ or haematopoietic stem cell allografts, CMV infections are common causes of morbidity and morta-lity.
In histology, the hallmark of CMV infection is the finding of intranuclear inclusions consistent with the virus. CISH-based diagnosis of CMV infection has the advan-tage over other methods in that it permits unequivocal localization of CMV genomes in cells and thereby obviates the risk of false positive results due to laboratory or clinical contamination.

Probe Description
The ZytoFast ® CMV Probe is directed against the sequence of the b2.7 gene, the most abundantly transcribed early CMV gene. The probe consists of  CMV-specific oligonucleotides, Biotin or Dig-oxigenin labeled by using the unique ZytoFast ® HighTag System providing improved signal intensity. In addition to the detection of CMV at the DNA level, the CMV Probe will also allow detection of the b2.7 RNA, which is expressed during all stages of infection.

Results
Due to the detection of CMV DNA as well as of the abundantly transcribed b2.7 RNA, a positive reactivity for cytomegalo-virus (CMV) in the target cells is indicated by a cytoplasmic and/or nuclear staining pattern. Depending on the detection che-mistry that is used, colored precipitates, which can be clearly distinguished from the background, will be dark violet-blue when using NBT/BCIP as substrate, strong red when using AEC, dark brown when using DAB, green when using HRP-Green,or strong red when using Permanent Red


采购指南

1、ZytoFast CMV-CISH Probes
      Biotin-labeled

T-1013-400 ZytoFast CMV Probe  Biotin 40 (400 μl)Related Products

相关产品：
T-1070-40 ZytoFast CISH Implementation Kit AP-NBT/BCIP 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Pepsin Solution, 4 ml; 20x Wash Buffer TBS, 2x 50 ml;AP-Streptavidin, 4 ml; NBT/BCIP, 4 ml

T-1071-40 ZytoFast CISH Implementation Kit HRP-AEC 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Pepsin Solution, 4 ml; 20x Wash Buffer TBS, 2x 50 ml;HRP-Streptavidin, 4 ml; AEC Solution, 4 ml


2、ZytoFast CMV-CISH Probes
      Digoxigenin-labeled

T-1113-400 ZytoFast CMV Probe  Digoxigenin 40 (400 μl)

相关产品：
T-1061-40 ZytoFast PLUS CISH Implementation Kit AP-NBT/BCIP       40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1  ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;  20x Wash Buffer TBS, 50 ml; Rabbit-anti-DIG, 4 ml; Anti-Rabbit-AP-Polymer, 4 ml; NBT/BCIP, 4ml; Nuclear Red Solution, 20 ml; Mounting Solution (alcoholic), 4 ml

T-1062-40 ZytoFast PLUS CISH Implementation Kit HRP-AEC        40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;  20x Wash Buffer TBS, 4x 50 ml; Mouse-anti-DIG, 4 ml; Anti-Mouse-HRP-Polymer, 4 ml; AEC Solution, 4 ml; Nuclear Blue Solution, 4 ml; Mounting Solution (aqueous), 4 ml

T-1063-40 ZytoFast PLUS CISH Implementation Kit HRP-DAB       40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;  20x Wash Buffer TBS, 4x 50 ml; Mouse-anti-DIG, 4 ml; Anti-Mouse-HRP-Polymer, 4 ml; DAB Solution A, 0.3 ml; DAB Solution B, 10 ml; Nuclear Blue Solution, 4 ml; Mounting Solution (alcoholic), 4 ml

T-1073-40 ZytoFast PLUS CISH Implementation Kit HRP-HRP-Green        40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;  20x Wash Buffer TBS, 4x 50 ml; Mouse-anti-DIG, 4 ml; Anti-Mouse-HRP-Polymer, 4 ml; HRP-Green-Solution A, 0.8 ml; HRP-Green-Solution B, 15 ml; Mounting Solution (alcoholic), 4 ml

T-1151-40 ZytoFast PLUS CISH Implementation Kit AP-Permanent Red 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;20x Wash Buffer TBS, 4x 50 ml; Rabbit-anti-DIG, 4 ml; Anti-Rabbit-AP-Polymer, 4 ml; Permanent Red Solution A, 0.2 ml; Permanent Red Solution B, 14 ml; Mayer’s Hematoxylin Solution, 20 ml;Mounting Solution (alcoholic), 4 ml