Background
The ZytoFast ® Ig-kappa/lambda-CISH System is designed for the detection of Ig-kappa (k) and/or Ig-lambda (l) light chain mRNA in paraffin-embedded tissue sections or cell samples.
B-cells (a.k.a. B lymphocytes) develop from lymphoid stem cells in the bone marrow.Each clone of B-cells expresses a unique antibody molecule, composed of 2 identical heavy and 2 identical light chains, the latter either of k or l type. Determination of kappa-to-lambda ratio is useful to distinguish between neoplastic and reactive lymphoid proliferations. Polyclonal expression of k or l light chains is considered to reflect a reactive hyperplasia in contrast
to the monoclonal expression in malignant lymphoma, the most common hematologic
malignancy encountered in the Western world. Whereas detection of Ig-k and Ig-l by immunohistochemistry often results in excessive background staining, in situ hybridization
has the advantage of a virtually background-free signal, allowing a safe and simple analysis of the clonality of a given lymphocyte population.

Probe Description
ZytoFast ® human Ig-kappa Probe is directed against mRNA sequences encoding k light chain constant regions of human immunoglobulins.
The ZytoFast ® human Ig-lambda Probe is directed against mRNA sequences encoding l light chain constant regions of human immunoglobulins.
The ZytoFast ® human Ig-kappa/Ig-lambda Probe is a probe mixture consisting of a Digoxigenin labeled Ig-k mRNA specific probe and a Biotin labeled Ig-l mRNA specific probe. All probes are tagged by using the unique ZytoFast ® HighTag System providing improved signal intensity.

Results
A positive reactivity in the target cells is indicated by cytoplasmic staining. Depending on the detection chemistry that is used, colored precipitates, which can be clearly distinguished from the background,will be dark violet-blue when using NBT/BCIP as substrate, strong red when using AEC, dark brown when using DAB, green when using HRP-Green, or strong red when using Permanent Red.
Using the ZytoFast ® human Ig-kappa Probe, B-cells expressing antibodies with k light chains will result in cytoplasmic staining whereas Ig-l expressing B-cells are not stained.
Using the ZytoFast ® human Ig-lambda Probe, B-cells expressing antibodies with l light chains will result in cytoplasmic staining whereas Ig-k expressing B-cells are not stained.
Using the ZytoFast ® human Ig-kappa/Ig-lambda CISH Kit, B-cells expressing antibodies with k light chains will result in a red cytoplasmic staining and simultaneously Ig-l expressing B-cells will result in a dark violet-blue cytoplasmic staining.


采购指南：

1、ZytoFast lg-kappa/lambda Probes
      Biotin-labeled

T-1015-400 ZytoFast human lg-kappa Probe 40 (400 μl)
T-1016-400 ZytoFast human lg-lambda Probe 40 (400 μl)

相关产品：
T-1070-40 ZytoFast CISH Implementation Kit AP-NBT/BCIP 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Pepsin Solution, 4 ml; 20x Wash Buffer TBS, 2x 50 ml;
AP-Streptavidin, 4 ml; NBT/BCIP, 4 ml

T-1071-40 ZytoFast CISH Implementation Kit HRP-AEC 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Pepsin Solution, 4 ml; 20x Wash Buffer TBS, 2x 50 ml;
HRP-Streptavidin, 4 ml; AEC Solution, 4 ml


2、ZytoFast lg-kappa/lambda Probes
      Digoxigenin-labeled

T-1115-400 ZytoFast human lg-kappa Probe 40 (400 μl)
T-1116-400 ZytoFast human lg-lambda Probe 40 (400 μl)

相关产品：
T-1061-40 ZytoFast PLUS CISH Implementation Kit AP-NBT/BCIP 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;20x Wash Buffer TBS, 4x 50 ml; Rabbit-anti-DIG, 4 ml; Anti-Rabbit-AP-Polymer, 4 ml; NBT/BCIP, 4ml; Nuclear Red Solution, 20 ml; Mounting Solution (alcoholic), 4 ml

T-1062-40 ZytoFast PLUS CISH Implementation Kit HRP-AEC 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;20x Wash Buffer TBS, 4x 50 ml; Mouse-anti-DIG, 4 ml; Anti-Mouse-HRP-Polymer, 4 ml; AEC Solution, 4 ml; Nuclear Blue Solution, 20 ml; Mounting Solution (aqueous), 4 ml

T-1063-40 ZytoFast PLUS CISH Implementation Kit HRP-DAB 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;20x Wash Buffer TBS, 4x 50 ml; Mouse-anti-DIG, 4 ml; Anti-Mouse-HRP-Polymer, 4 ml; DAB Solution A, 0.3 ml; DAB Solution B, 10 ml; Nuclear Blue Solution, 20 ml; Mounting Solution (alcoholic), 4 ml

T-1073-40 ZytoFast PLUS CISH Implementation Kit HRP-HRP-Green 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;20x Wash Buffer TBS, 4x 50 ml; Mouse-anti-DIG, 4 ml; Anti-Mouse-HRP-Polymer, 4 ml; HRP-Green-Solution A, 0.8 ml; HRP-Green-Solution B, 15 ml; Nuclear Red Solution, 20 ml; Mounting Solution (alcoholic), 4 ml

T-1151-40 ZytoFast PLUS CISH Implementation Kit AP-Permanent Red 40
Incl. DNA (+)-Control Probe, 0.1 ml; DNA (-)-Control Probe, 0.1 ml; RNA (+)-Control Probe, 0.1 ml; RNA (-)-Control Probe, 0.1 ml; Heat Pretreatment Solution EDTA, 500 ml; Pepsin Solution, 4 ml;20x Wash Buffer TBS, 4x 50 ml; Rabbit-anti-DIG, 4 ml; Anti-Rabbit-AP-Polymer, 4 ml; Permanent Red Solution A, 0.2 ml; Permanent Red Solution B, 14 ml; Mayer’s Hematoxylin Solution, 20 ml;Mounting Solution (alcoholic), 4 ml


3、ZytoFast lg-kappa/lambda Probes
      Biotin/Digoxigenin-labeled

T-1017-400 ZytoFast human lg-kappa/lg-lambda Probe 40 (400 μl)

相关产品：
T-1005-10 ZytoFast human lg-kappa/lg-lambda CISH Kit 10
Incl. Ig-kappa/lg-lambda Probe (Digoxigenin/Biotin labeled), 0.1 ml; RNA (+)-Control Probe (Biotin labeled), 0.1 ml; RNA (-)-Control Probe (Biotin labeled), 0.1 ml; Pepsin Solution, 1 ml;
20x Wash Buffer TBS, 50 ml; HRP-anti-Digoxigenin, 1 ml; AP-Streptavidin, 1 ml; AEC Solution, 1 ml; NBT/BCIP, 1 ml

T-1005-40 ZytoFast human lg-kappa/lg-lambda CISH Kit 40
Incl. Ig-kappa/lg-lambda Probe (Digoxigenin/Biotin labeled), 0.4 ml; RNA (+)-Control Probe (Biotin labeled), 0.1 ml; RNA (-)-Control Probe (Biotin labeled), 0.1 ml; Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 2x 50 ml; HRP-anti-Digoxigenin, 4 ml; AP-Streptavidin, 4 ml; AEC Solution, 4 ml; NBT/BCIP, 4 ml