生物反恐诊断试剂盒
英文名称： 生物反恐快速检测试剂盒
型号:null    产品货号： 生物反恐快速检测试剂盒
价格:请致电：010-57128832,18610462672
品牌: 中国
试剂级别： 试剂级

 

编号 产品名称 规格 注册文号
A1122 炭疽抗体快速检测试剂 10T 国食药监械（准）字2012第3400474号
A1112 炭疽抗原快速检测试剂 10T 国食药监械（准）字2012第3400474号
A0922 鼠疫抗体快速检测试剂 10T 国食药监械（准）字2012第3400470号
A0912 鼠疫抗原快速检测试剂 10T 国食药监械（准）字2012第3400470号
A1222 土拉热抗体快速检测试剂 10T 国食药监械（准）字2012第34004741号
A1212 土拉热抗原快速检测试剂 10T 国食药监械（准）字2012第3400471号
A1022 布鲁氏菌抗体快速检测试剂 10T 国食药监械（准）字2012第3400472号
A1012 布鲁氏菌抗原快速检测试剂 10T 国食药监械（准）字2012第3400472号
A1322 鼻疽抗体快速检测试剂 10T 国食药监械（准）字2012第3400476号
A1312 鼻疽抗原快速检测试剂 10T 国食药监械（准）字2012第3400476号
A1422 类鼻疽抗体快速检测试剂 10T 国食药监械（准）字2012第3400477号
A1412 类鼻疽抗原快速检测试剂 10T 国食药监械（准）字2012第3400477号
A1512 A型肉毒毒素快速检测试剂 10T 国食药监械（准）3400473号
A1612 B型金黄色葡萄球菌肠毒素快检试剂 10T 国食药监械（准）3400475号
 

ont-kerning: 0pt" lang=EN-US>Benefits

The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled.

Product Description

Sensitivity and specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

1.       DIG-High Prime, 5x conc., 50 µl

2.       DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)

3.       DNA Dilution Buffer, 3 x 1 ml

4.       Anti-digoxigenin-AP Conjugate, 100 µl

5.       NBT/BCIP Stock Solution, conc., 6 x 1 ml

6.       Blocking Solution, 10x conc., 4 x 100 ml

DIG Easy Hyb Granules, 4 x 100 ml

DIG高效DNA标记及检测Starter Kit II说明书

DIG高效DNA标记及检测Starter Kit II

DIG High Prime DNA Labeling and Detection Starter Kit II：Kit for chemiluminescent detection with CSPD, ready-to-use

对于需要使用化学发光法检测的用户，我公司现提供了此新的DIG标记及检测试剂盒。

与上述的StarterKit I不同，此新的StarterKit II所含的底物为CSPD，但它同样有以下特点：

●能对10×10cm印迹膜作12个标记反应及24个检测反应，所以特别适合初用者及需处理较少量样品的用户使用。
●包含DIG高效标记试剂，令标记效率更高，操作更方便。
●包括DIG定量/对照试纸条及NBT/BCIP浓缩液，令您更易估计探针浓度，从而取得理想的信噪比。

产品号

产品规格

厂商

价格

1585614

11585614910

Kit：1 kit (12 labeling reactions and 24 detection reactions)

Roche

4719

DIG-High Prime DNA Labeling and Detection Starter Kit II

Application

DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.

Product Description

Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

1.       DIG-High Prime, 5x conc., 50 µl

2.       DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)

3.       DNA Dilution Buffer, 3 x 1 ml

4.       Anti-digoxigenin-AP Conjugate, 50 µl

5.       CSPD, ready-to-use, 50 ml

6.       Blocking Solution, 10x conc., 4 x 100 ml

7.       DIG Easy Hyb Granules, 4 x 100 ml