Description: ESGRO Complete Clonal Grade Medium
Product Overview:
ESGRO Complete™ Clonal Grade Medium is a defined serum-free medium optimized to grow and maintain undifferentiated mouse embryonic stem cells in the absence of serum and feeder cells. Intended for mouse embryonic stem (ES) cell expansion and growth at clonal density in serum-free conditions. Cells should maintain germ line competency.
Key Applications: Stem Cell Culture
Application Notes:
Suitable for ES cell derivation, transfection and selection, in addition to general cell passaging and growth.
Media can be used on feeder dependent/independent ES cell lines.
Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells using ESGRO Complete products. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete™ Accutase (Cat # SF006)
ESGRO Complete™ Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete™ Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Reagent Type: Cell Culture Media
Media Type: Cell Culture Media
Presentation: Complete formulation.
Contains self-renewal factors, no need to add LIF.
pH 7.1-7.3
Quality Assurance:
QC PROTOCOL:
Low density plating assay:
1. Plate feeder independent early passage ES cells onto gelatin coated 10 cm plastic tissue culture dish at 1000 cells/10 cm dish in 10-20 mL of Clonal Grade Medium.
2. Change medium every 2-4 days.
3. Monitor for clonal growth with alkaline phosphatase stain.
Colony formation should occur over 4-6 days. The majority of colonies should show no signs of differentiation.
Protein: ESGRO
Storage Conditions:
Store at -20°C. Refer to lot expiration date on label. After thawing, store at 4°C for one month. DO NOT REFREEZE.
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