产品名称
Anti-Ki67抗体
描述
兔多克隆抗体to Ki67
特异性
Ab15580 is batch tested in ICC/IHC. Some customers have successfully used ab15580 in Western Blot. Higher molecular weight proteins like ki67 may be more difficult to detect in WB. We recommend several potential optimisation steps: loading higher amounts of protein (20 ug and above), using lower percentage gels and/or Tris-Acetate gels, increasing antioxidant to maintain protein reduction, decreasing methanol and increasing SDS in the transfer buffer and increasing time and voltage of transfer. Larger proteins can be subject to degradation more than smaller proteins so lower molecular weight bands may be present. For further information or support please contact our Scientific Support Team.
经测试应用
适用于: IHC - Wholemount, IHC-P, IHC-FrFl, Flow Cyt, IHC-Fr, ICC/IF, ICC, IHC-FoFrmore details
种属反应性
与反应: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Dog, Human, Pig, Indian muntjac, Monkey, Chinese hamster, Common marmoset, Syrian hamster
免疫原
Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67.
阳性对照
ICC-IF: Ki67 wildtype and knockout HAP1 cells IF: mouse (P0) olfactory bulb, MEF1 IHC-Fr: mouse P7 brain sections IHC-P: mouse spleen
形式Liquid
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
Batch dependent within range: 100 µg 浓度为 0.7 - 1 mg/ml
纯度
Immunogen affinity purified