产品名称
Anti-Histone H3 (tri methyl K4)抗体- ChIP Grade
描述
兔多克隆抗体to Histone H3 (tri methyl K4) - ChIP Grade
经测试应用
适用于: ChIP/Chip, PepArr, ICC, ChIP, WB, IHC-Fr, IP, CHIPseq, Flow Cyt, ICC/IF, IHC-Pmore details
种属反应性
与反应: Mouse, Rat, Rabbit, Human, Pig, Saccharomyces cerevisiae, Tetrahymena, Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish, Trypanosoma cruzi, Common marmoset, Rice
预测可用于: Cow, Indian muntjac, Oikopleura, Plants, Mammal
免疫原
Synthetic peptide within Human Histone H3 aa 1-100 (tri methyl K4) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available as ab92374)
阳性对照
WB: Calf thymus histone preparation. ICC/IF: HeLa cells. IHC-P: Normal human colon tissue.
常规说明
In immunofluorescence, a distinct property of tri methyl lysine 4 is its apparent ringing of regions that appear as nucleoplasmic holes. These represent the positions of splicing factor compartments, which often are easy to identify using only DNA stains in Indian muntjac fibroblasts. These splicing factor compartments are known to be preferentially associated with active genes and highly acetylated histone H3. The antibody, as expected, fails to stain heterochromatin (work by Kirk McManus, lab of Michael Hendzel).
The immunofluorescence results suggest this antibody is an exceptional euchromatin probe.
形式
Liquid
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
Batch dependent within range: 50 µg 浓度为 0.2 - 1 mg/ml
纯度
Immunogen affinity purified