支原体抑制剂溶液 英文名称: MIS |
型号:null 产品货号: 0563 |
价格:请致电:010-57128832,18610462672 |
品牌: 美国sciencell |
北京裕恒丰科技有限公司,已成功代理美国Sciencell公司细胞生物学产品十余年。多年来,裕恒丰一直秉承信誉至上的原则,在点滴中慢慢地提升企业信誉,树立企业形象,我们始终以热心的服务和专业的技术支持,为广大科研工作者提供了优质的细胞及分子生物学产品。在未来的发展中,我们将继续努力打造企业品牌,北京裕恒丰不仅希望您能聆听到我们的心声,更期待您能亲自体验我们的服务,北京裕恒丰欢迎您的光临。 温馨提示:ScienCell产品仅用于科研,不能用于人或动物以及体外诊断应用。如预购买产品请登陆ScienCell唯一官网(www.sciencellonline.com)寻找正规代理商,以确保您的实验顺利进行。 支原体抑制剂溶液 货号0563 产品介绍 Mycoplasma inhibitor solution is formulated with 10mg/ml ciprofloxacin. Ciprofloxacin belongs to the fluoroquinolone antibiotic family and is effective against mycoplasma, gram positive and gram negative bacteria [1, 2]. Ciprofloxacin inhibits bacterial DNA gyrase necessary for active and non-active growth phases [3]. Add fresh mycoplasma inhibitor solution to culture media and change every 3-4 days. A two week treatment should be sufficient to eliminate contamination. The effective working concentration ranges from 5-25 μg/ml; 10 μg/ml is ideal for most applications. The optimal concentration, however, should be determined for individual cell types and culture conditions. 参考文献 [1] Schmitt K, Daubener W, Bitter-Suermann D, and Hadding U. (1988) “A safe and efficient method for elimination of cell culture mycoplasmas using ciprofloxacin”. J Immunol Methods 109: 17-25. [2] Drexler HG and Uphoff CC. (2002) “Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention”. Cytotechnology 39: 75-90. [3] Drlica K and Zhao X. (1997) “DNA gyrase, topoisomerase IV, and the 4-Quinolones”. Microbiol Mol Biol Rev 61: 377-392.
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