英文名称： Monoclonal Anti-β-Actin antibody produced in mouse
Monoclonal Anti-β-Actin antibody produced in mouse
Monoclonal Anti-β-Actin antibody produced in mouse
clone AC-15, ascites fluid
DOWNLOAD MSDS (PDF)Synonym: Monoclonal Anti-β-Actin
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Properties
| Related Categories | A, AC-AC, Actin Antibodies, Alphabetical Index,Antibodies, |
| species reactivity | canine, guinea pig, Hirudo medicinalis, feline, pig, mouse, carp, chicken, sheep, rabbit, rat, human, bovine |
| does not react with | Dictyostelium discoideum |
| application(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts | |
| western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts | |
| clone | AC-15, monoclonal |
| antibody form | ascites fluid |
| isotype | IgG1 |
| mol wt | antigen mol wt 42 kDa |
| contains | 15 mM sodium azide |
| shipped in | dry ice |
| storage temp. | −20°C |
| Gene Information | human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822) |
Description
Immunogen
slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Al
General description
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
The antibody can be used for staining of acetone-fixed frozen sections, EM preparations, and microinjection experiments. B5, ethanol, methacam, or Bouin′s solutions can be used as fixatives. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-nonmuscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues.
Application
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.1
Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.
Other Notes
To view an Actin antibody selection guide, please visitwww.sigmaaldrich.com/acti
Frequently Asked Questions
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