| Application | Dilutions |
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry | 1:2000 |
| Immunofluorescence | 1:400 |
| Flow Cytometry | 1:6400 |
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.
Species Reactivity: Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology: Dog
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Data Sheets & Documentation
