美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。

髓核是椎间类似凝胶样的物质。它的作用是在压缩负载的情况下全面地调节液压。髓核由软骨样的细胞，胶原纤维和蛋白多糖组成。髓核细胞存在于维管联结有限和通过无氧糖酵解产生能量的环境中。髓核细胞表达缺氧诱导因子-1以回应氧压和调节糖酵解。它们在胎儿和童年时候表达细胞角蛋白和波形蛋白：在细胞培养中分泌白细胞介素-1，-6，-10和粒细胞-巨噬细胞共刺激因子。髓核细胞体外培养为研究椎间盘退变，组织工程提供了细胞学及分子生物学机制。

      12.骨骼细胞系统
     4600 人颅骨造骨细胞 (HCO) ( 5×105 )
     4700 人滑膜细胞（HS）( 5×105 )
     4800 人髓核细胞(HNPC)(5×105)

人髓核细胞提取于人椎间盘髓核，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过角蛋白及波形蛋白抗体免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。

Description

Nucleus pulposus is the jelly-like substance in the middle of the intervertebral disc. It functions to distribute hydraulic pressure in all directions within each disc under compressive loads. The nucleus pulposus consists of collagen fibrils, proteoglycan aggrecans and nucleus pulposus cells. The nucleus pulposus cells reside in an environment that has a limited vascular supply and generate energy through anaerobic glycolysis. The cells synthesize collagen II and X; express hypoxia-inducible factor-1 [1]; and secrete interleukins-1, -6, and -10 and granulocyte-macrophage colony-stimulating factor in culture [2]. Mechanical stress on nucleus pulposus cells promotes cell proliferation [3]. The nucleus pulposus cell culture provides an in vitro model for the study of cellular and molecular events involved in disc degeneration, tissue engineering and cell therapy for spine disc disorders.

HNPC from ScienCell Research Laboratories are isolated from nucleus pulposus of human intervertibral disc. HNPC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HNPC are characterized by immunofluorescent method with antibodies to fibronectin and vimentin. HNPC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HNPC are guaranteed to further expand for 15 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Nucleus Pulposus Cell Medium (NPCM, Cat. No. 4801) for the culturing of HNPC in vitro.

Product Use

HNPC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1]. A Agrawal, A Guttapalli, S Narayan, TJ. Albert, IM. Shapiro, and MV. Risbud (2007) Normoxic stabilization of HIF-1? drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk. Am J Physiol Cell Physiol 293: C621-C631.
[2]. K Torsten, N Thomas, G Christoph, G Tatiana (2005) Human Anulus Fibrosis and Nucleus Pulposus Cells of the Intervertebral Disc: Effect of Degeneration and Culture System on Cell Phenotype. Spine 30(24):2743-2748.
[3]. M Takuji, K Mamoru, K Koichi, T Toru, T Tetsuya (1999) Cyclic Mechanical Stretch Stress Increases the Growth Rate and Collagen Synthesis of Nucleus Pulposus Cells In Vitro. Spine 24(4):315-319.