上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理：

       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。
 

请登录Sciencell公司官方网站（www.sciencell-s.com或www.sciencellonline.com）以确保购买正规公司产品。   

                
人肝窦内皮细胞说明：
货号： 5000

内皮细胞具有多种生理功能，并且是许多病理过程的中心环节。肝脏含有两种不同类型的内皮细胞：血管内皮细胞和肝窦内皮细胞。肝窦内皮细胞是具有类似树突状细胞特殊表型的微血管内皮细胞，并能作为CD4+-T细胞的抗原呈递细胞。因此，肝窦内皮细胞表现为一种新型的器官定植 “非专业”抗原呈递细胞，在肝脏局部免疫调控和诱导免疫耐受中发挥作用。肝脏的微环境系统，如门静脉系统成分和来自肝窦细胞的可溶性介质，严密的控制着SEC的抗原呈递作用，以避免出现由免疫介导的损伤。SEC表达特征性的表面受体，并且在形态和代谢上与大血管内皮细胞明显不同。有报道显示在肝再生过程中，SEC是能对环境区域刺激做出快速的局部的反应孔隙的动态调节器。由于SEC在肝窦中的重要位置，使得SEC机能异常和结构改变将会对整个肝脏造成广泛的影响。     人肝窦内皮细胞(HHSEC)提取于人肝组织，提取纯化之后立即冻存。每管含有细胞数>5×105 cells/ml，此细胞通过vWF/Factor VIII 和P-CAM (CD31)免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。 推荐培养基:(ECM, Cat. No. 1001) Description   Endothelial cells play multiple physiological functions and are central to many pathological processes. The liver contains two distinct endothelial cell types: vascular and sinusoidal. Sinusoidal endothelial cells (SEC) are microvascular endothelial cells with a unique phenotype reminiscent of dendritic cells and a unique function as antigen-presenting cells for CD4+ T cells. Thus, SEC represent a new type of organ-resident "non-professional" antigen-presenting cell that appears to be involved in the local control of the immune response and the induction of immune tolerance in the liver [1]. The hepatic microenvironment, i.e. portal venous constituents and soluble mediators from sinusoidal cell populations, tightly control antigen presentation by SEC to avoid immune-mediated damage. SEC express well-characterized surface receptors and differ morphologically and metabolically from large-vessel endothelia [3]. It has reported that SEC are dynamic regulators of porosity that respond rapidly and locally to environmental zonal stimuli during liver regeneration [2]. Due to its strategic position in the liver sinusoid, SEC dysfunction and structural alterations have far-reaching repercussions for the whole liver. HHSEC from ScienCell Research Laboratories are isolated from human liver. HHSEC are cryopreserved immediately after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HHSEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM). HHSEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHSEC are guaranteed to further expand for 15 population doublings in the conditions provided by ScienCell Research Laboratories. Recommended Medium   It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HHSEC. Product Use   HHSEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage   Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. Shipping   Dry ice. Reference   [1] Limmer A, Knolle PA. (2001) Liver sinusoidal endothelial cells: a new type of organ-resident antigen-presenting cell. Arch Immunol Ther Exp (Warsz) 49 Suppl 1:S7-11. [2] Wack KE, Ross MA, Zegarra V, Sysko LR, Watkins SC, Stolz DB. (2001) Sinusoidal ultrastructure evaluated during the revascularization of regenerating rat liver. Hepatology 33(2):363-78. [3] Gervaz P, Scholl B, Mainguene C, Poitry S, Gillet M, Wexner S. (2000) Angiogenesis of liver metastases: role of sinusoidal endothelial cells. Dis Colon Rectum 43(7):980-6.

      13.肝脏细胞系统
    5000 人肝窦内皮细胞 (HHSEC) ( 5×105 )
    5100 人肝内胆管上皮细胞 (HIBEC)( 5×105 )
    5200 人肝细胞 (HH) (1×106 )
    5300 人肝星形细胞 (HHSteC)( 5×105 )