上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理：

       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。
 

请登录Sciencell公司官方网站（www.zqxzbio.com或www.sciencellonline.com）以确保购买正规公司产品。   

                
人肝细胞说明：
货号：5200

哺乳动物的肝脏完成许多重要生理过程。不管在生理还是病理条件下，它都是能量代谢、外源性化学物质生物转化和血浆蛋白合成最重要的器官。原代培养人肝细胞是很好的实验模型，在过去和现在都是研究肝脏特定功能的重要工具。用繁殖的人肝细胞进行细胞移植、基因治疗和用于生物人工肝支持系统的肝细胞培养都在研究中。在合适的培养条件下，培养的人类肝细胞增殖并保持已分化的肝细胞功能，比如血清蛋白的合成。     人肝细胞(HH)提取于人肝脏组织，于原代末期冻存。每管含有细胞数>1× 106cells/ml，此细胞通过albumin, Cytokeratin-18 and Vimentin免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。 推荐培养基:(HM, Cat. No. 5201) Description   The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, biotransformation of xenobiotics, and synthesis of plasma proteins under physiological and pathophysiological conditions [1]. Primary culture of human hepatocyte appears to be a suitable experimental model for the study of liver specific function, have been and still is an important tool. Propagation of human hepatocytes for cell transplantation, gene therapy, and culture of hepatocyte in bioartificial liver support systems is now under investigation [2]. In appropriate culture condition, cultured human hepatocytes proliferate and maintain differentiated hepatocyte function such as the synthesis of serum proteins [3]. HH from ScienCell Research Laboratories are isolated from human liver. HH are cryopreserved immediately after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HH are characterized by immunofluorescent method with antibodies to albumin, cytokeratin-18 and vimentin. HH are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HH is not recommend for expanding or long term cultures since the cells do not proliferate in culture. Recommended Medium   It is recommended to use Hepatocyte Medium (HM, Cat. No. 5201) for the culturing of HH in vitro. Product Use   HH are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage   Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. Shipping   Dry ice. Reference   [1] Runge, D., Michalopoulos, G. K., Strom, S. C. and Runge, D. M. (2000) Recent advances in human hepatocyte culture systems. Biochem. Biophysi. Res. Comm. 274:1-3. [2] Chen, H. L., Wu, H. L., Fon, C. C., Chen, P. J., Lai, M. Y. and Chen, D. S. (1998) Long-term culture of hepatocytes from human adults. J. Biomed. Sci. 5:435-440. [3] Okamoto, M., Ishida, Y., Keogh, A. and Strain, A. (1998) Evaluation of the function of primary human hepatocytes co-cultured with the human hepatic stellate cell (HSC) line LI90. Int. J. Artif. Organs 21:353-359.

      13.肝脏细胞系统
    5000 人肝窦内皮细胞 (HHSEC) ( 5×105 )
    5100 人肝内胆管上皮细胞 (HIBEC)( 5×105 )
    5200 人肝细胞 (HH) (1×106 )
    5300 人肝星形细胞 (HHSteC)( 5×105 )