上海中乔生物科技有限公司是美国Sciencell公司中国一级代理: 美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售8年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。 ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。 请登录Sciencell公司官方网站(www.zqxzbio.com或www.sciencellonline.com)以确保购买正规公司产品。  人肾小球内皮细胞说明: 货号:4000 肾小球内皮细胞是一类特殊微血管类型的细胞,能够调节肾小球过滤。它是组成过滤屏障内壁的重要部分,与肾小球的病理生理过程有关。肾小球内皮细胞能合成必要的生物活性分子,它的这种基本活性会在致炎和致血栓物质的刺激下慢慢增强。肾小球内皮细胞的受损显著影响着肾小球疾病的进展和修复过程。当肾小球损害严重时,内皮受损无法新生血管,硬化代替受损区域。肾小球内皮细胞受损后不可避免地会影响系膜细胞和上皮细胞并有可能通过它们的相互作用改变肾脏病的进展。因为这类细胞培养、克隆、增殖都比较困难,其生物学特点至今知之甚少。 9.肾脏细胞系统 4000 人肾小球内皮细胞 (HRGEC)( 5×105 ) 4100 人肾近端小管上皮细胞(HRPTEpiC) 4110 人肾皮质上皮细胞(HRCEpiC)( 5×105 ) 4120 人肾脏上皮细胞(HREpiC)( 5×105 ) 4200 人肾系膜细胞 (HRMC)(1×106 ) 人肾小球内皮细胞(HRGEC)提取于人肾组织,原代冻存。每管含有细胞数>5×105 cells/ml,此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。 推荐培养基:(ECM, Cat. No. 1001) Description Renal glomerular endothelial cells (GEC) are a specialized microvascular cell type involved in the regulation of glomerular ultrafiltration. They form the inner part of the filtration barrier and are involved in pathophysiological processes in the glomerulum [1]. They constitutively synthesize bio-active molecules, to which this basal activity can be chronically augmented by various inflammatory and thrombotic agents [2]. GEC injury exerts significant influences on the progression and repair process of glomerular disease. When the glomerular lesion is severe, angiogenesis is prevented due to endothelial cell injury, with subsequent sclerosis taking place in the impaired region [3]. These glomerular endothelial cell injuries inevitably affect mesangial and epithelial cells and presumably modify the progression of renal disease by reciprocally interacting with them [4]. Because of difficulties associated with the culture, cloning and propagation, the biological properties of these cells remain largely unknown. HRGEC from ScienCell Research Laboratories are isolated from human kidneys. HRGEC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRGEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM), and by the formation of microtublar structure in vitro. HRGEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRGEC are guaranteed to expand beyond 15 population doublings at the conditions specified by ScienCell Research Laboratories. Recommended Medium It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HRGEC in vitro. Product Use HRGEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. Shipping Dry ice. Reference [1] Nangaku, M., Shankland, S. J., Couser, W. G. and Johnson, R. J. (1998) A new model of renal microvascular injury. Curr Opin Nephrol Hypertens 7(4):457-62. [2] Kester, M., Nowinski, R. J., Holthofer, H., Marsden, P. A. and Dunn, M. J. (1994) Characterization of platelet-activating factor synthesis in glomerular endothelial cell lines. Kidney Int 46(5):1404-12. [3] Lee, L. K., Meyer, T. W., Pollock, A. S. and Lovett, D. H. (1995) Endothelial cell injury initiates glomerular sclerosis in the rat remnant kidney. J Clin Invest 96(2):953-64. [4] Yamanaka, N. and Shimizu, A. (1999) Role of glomerular endothelial damage in progressive renal disease. Kidney Blood Press Res 22(1-2):13-20. |