上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理：

       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售8年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严15的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。
 

请登录Sciencell公司官方网站（www.zqxzbio.com或www.sciencellonline.com）以确保购买正规公司产品。   

人角膜上皮细胞说明：
货号：6510

人角膜是唯一的无血管的组织，具有透明的特性和合成许多蛋白的功能。角膜包含三个不同的细胞层，外层是上皮细胞，内层是内皮细胞，中间层基质层（角膜细胞）。角膜上皮细胞能参与先天性免疫，它能感应病原体存在并发出信号从而激活角膜防御系统。还能高效表达醛脱氢酶，防止UV-和4-羟基壬烯醛对细胞造成伤害。角膜内皮细胞增殖是由细胞因子控制的，比如说表皮生长因子，它能激活上皮细胞深层的同源受体。体外培养角膜内皮细胞已经可以替代检测动物眼睛的产物而用于毒理学检测和人角膜上皮细胞分化的分子机制的研究。     人角膜上皮细胞(HCEpiC)提取于人角膜组织，于原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过Cytokeratine-18 和 -19免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到10倍增。 推荐培养基:(FM, Cat. No. 6511) Description   The cornea is a unique tissue for two reasons: the transparency function and the synthesis of most of its proteins due to the cornea being an avascular tissue. The cornea consists of three distinct cell layers, the outer epithelium, the inner endothelium and the central stroma (the keratocytes). The corneal epithelium plays a role in the innate immune response by sensing the presence of pathogens and providing signals that activate the corneal defense system [1]. It also highly expresses aldehyde dehydrogenase to protect against UV- and 4-hydroxynonenal-induced cellular damage [2]. Corneal epithelial basal cell proliferation is controlled by a host of cytokines, e.g., epidermal growth factor, that activate their cognate receptors in the deeper layers of the epithelium [3]. Instead of testing products in the eyes of animals, cultured corneal epithelial cells could be used for toxicology tests in in vitro models and for studying the molecular mechanisms involved in regulating human corneal epithelial cell differentiation. HCEpiC from ScienCell Research Laboratories are isolated from human cornea. HCEpiC are cryopreserved from passage one culture. Each vial contains >5 x 10^5 cells in 1 ml volume. HCEpiC are characterized by their cobble stone morphology in serum-free culture and immunofluorescent method with antibody to CK-18 and -19. HCEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCEpiC are guaranteed to further expand for 10 population doublings at the condition provided by ScienCell Research Laboratories. Recommended Medium   It is recommended to use Corneal Epithelial Cell Medium (CEpiCM, Cat. No. 6511) for the culturing of HCEpiC in vitro. Product Use   HCEpiC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage   Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. Shipping   Dry ice. Reference   [1] Zhang, J., Wu, X. Y. and Yu, F. S. (2005) Inflammatory response of corneal epithelial cells to pseudomonas aeruginosa infection. Curr. Eye Res. 30:527-534. [2] Pappa, A., Brown, D., Koutalos, Y., Degregori, J., White, C. and Vasiliou, V. (2005) Human aldehyde dehydrogenase 3A1 inhibits proliferation and promotes survival of human corneal epithelial cells. J. Biol. Chem. 280:27998-28006. [3]. Yang, H., Mergler, S., Sun, X., Wang, Z, Lu, L., Bonanno, J. A., Pleyer, U. and Reinach, P. S. (2005). TRPC4 knockdown suppresses EGF-induced store operated channel activation and growth in human corneal epithelial cells. J Biol Chem.

       

15.视觉细胞系统
    6510 人角膜上皮细胞 (HcorF)( 5×105 )
    6520 人角膜成纤维细胞 (HcorF)( 5×105 )
    6530 人视网膜内皮细胞 (HREC)( 5×105 )
    6540 人视网膜色素上皮细胞(HRPEpiC)(5×105 )
    6550 人晶状体上皮细胞 (HLEpiC)( 5×105 )
    6560 人虹状体上皮细胞 (HIPEpiC)( 5×105 )
    6570 人结膜纤维原细胞（HConF）（5×105 )
    6580 人非色素睫状上皮细胞 (HNPCEpiC)( 5×105 )
    6590 人小梁网细胞(HTMC)(5×105)