上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理: 美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。 ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。 请登录Sciencell公司官方网站(www.zqxzbio.com或www.sciencellonline.com)以确保购买正规公司产品。  人主动脉内皮细胞说明: 货号:6100 内皮细胞构成了血管和其下组织的交界面。内皮细胞功能的变化是动脉粥样硬化的发病机理中的关键因素。内皮细胞能合成分泌凝血系统和纤溶系统中的活化因子和抑制因子,同时也能合成和分泌影响着血小板粘附和聚集的某些介质。内皮细胞还可以释放具有调控细胞增殖和血管壁张力功能的分子。人主动脉内皮细胞能生成诸如t-PA 和 PAI-1等抗凝和血栓形成因子;在TNF-alpha的调节下,人主动脉内皮细胞能改变其生长特性,产生GM-CSF等细胞因子,在细胞表面表达ICAM-1,并产生大量的NO和内皮素。许多内皮水平的过程多可以通过内皮细胞的体外培养进行研究,因此,HAEC非常适用于研究和认识血管病变和衰老的分子机理。 人主动脉内皮细胞(HAEC)提取于人主动脉组织,原代冻存。每管含有细胞数>5×105 cells/ml,此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。 推荐培养基:(ECM, Cat. No. 1001) | | Description Endothelial cells constitute the natural interface between the blood and the underlying tissue. Changes in endothelial cell function appear to play a key role in the pathogenesis of atherosclerosis. Endothelial cells synthesize and secrete activators as well as inhibitors of both the coagulation system and the fibrinolysis system in addition to mediators that influence the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that control cell proliferation and modulate vessel wall tone [1]. Human aortic endothelial cells (HAEC) produce antithrombotic and thrombotic factors such as t-PA and PAI-1 and respond to TNF-alpha by modifying growth characteristics, producing cytokines such as GM-CSF, expressing ICAM-1 on the surface and producing large amounts of nitric oxide and endothelin [2]. Many of the endothelial processes can be studied in vitro using cultured cells, and HAEC will surely be very useful to studying and understanding the molecular mechanisms involved in many vascular alteration pathologies as well as in the aging process. HAEC from ScienCell Research Laboratories are isolated from human aorta. HAEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HAEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HAEC in vitro. Product Use HAEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. Shipping Dry ice. Reference [1] Elhadj, S. and Forsten, K. E. (2001) Chronic shear stress affects bovine aortic endothelial cell secreted proteoglycan production in vitro. Bioengineering Conference 50:767-768. [2] Donnini, D., Perrella, G., Stel, G., Ambesi-Impiombato, F. S., Curcio, F. (2000) A new model of human aortic endothelial cells in vitro. Biochimie 82:1107-14. | 14.心脏细胞系统 6000 人心脏微血管内皮细胞 (HCMEC)( 5×105 ) 6100 人主动脉内皮细胞 (HAEC)( 5×105 ) 6110 人主动脉平滑肌细胞 (HASMC)( 5×105 ) 6200 人心肌细胞 (HCM) (1×106 ) 6210 ***心肌细胞 (HCMa) (1×106 ) 6300 人心脏成纤维细胞( HCF) ( 5×105 ) 6310 ***心室成纤维细胞 (HCFav)( 5×105 ) 6320 ***心房成纤维细胞 (HCFaa)( 5×105 ) |