人肠平滑肌细胞
英文名称: Human Intestinal Smooth Muscle Cells
型号:null    产品货号: XY2910
价格:请致电:010-57128832,18610462672
品牌: 中国/美国
试剂级别: 细胞培养级

人肠平滑肌细胞Cell Specification
Smooth muscle contraction is the fundamental event in gastrointestinal motion. Although many
of the biochemical mechanisms underlying the excitation-contraction coupling are not yet
defined, it is known that cytosolic Ca2+ is the essential component in the coupling phenomenon.
Inflammation of the human intestine causes increased levels of smooth muscle-specific actin,
which in turn promotes the thickening of the smooth muscle layers. The increased smooth
muscle actin may affect force production and further demonstrates the plasticity of smooth
muscle cells in the inflamed intestine [1]. Studies also show that human intestinal smooth muscle
cells (HISMC) respond to IL-1beta and TNF-alpha stimulation by releasing IL-6, which may
significantly contribute to the overall systemic inflammatory response [2]. The availability of
HISMC makes it more feasible to study the contractile and proliferative tissue responses of
human intestinal smooth muscle.
HISMC from ScienCell Research Laboratories are isolated from human intestine. HISMC are
cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105
cells in 1 ml
volume. HISMC are characterized by immunofluorescence with antibodies specific to -smooth
muscle actin and desmin. HISMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria,
yeast and fungi. HISMC are guaranteed to further expand for 15 population doublings under the
conditions provided by ScienCell Research Laboratories.
Recommended Medium
人肠平滑肌细胞It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. #1101) for culturing
HISMC in vitro.
Product Use
HISMC are for research use only. They are not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and
keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
References
[1] Blennerhassett MG, Bovell FM, Lourenssen S, McHugh KM. (1999) “Characteristics of inflammation-induced
hypertrophy of rat intestinal smooth muscle cell.” Dig Dis Sci. 44(7):1265-72.
[2] Ng EK, Panesar N, Longo WE, Shapiro MJ, Kaminski DL, Tolman KC, Mazuski JE. (2003) “Human intestinal
smooth muscle cells are potent producers of IL-6.” Mediators Inflamm. 12(1):3-8.
Instructions for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37oC water bath and
return the cells to culture as quickly as possible with minimal handling!
Initiating the culture:
1. Prepare a poly-L-lysine-coated culture vessel (2 μg/cm2
, T-75 flask is recommended).
Add 10 ml of sterile water to a T-75 flask and then add 15 μl of poly-L-lysine stock
solution (10 mg/ml, Cat. #0413). Leave the vessel in a 37oC incubator overnight (or for a
minimum of one hour).
2. Prepare complete medium. Decontaminate the external surfaces of medium bottle and
medium supplement tubes with 70% ethanol and transfer them to a sterile field.
Aseptically transfer supplement to the basal medium with a pipette. Rinse the supplement
tube with medium to recover the entire volume.
3. Rinse the poly-L-lysine-coated vessel twice with sterile water and then add 15 ml of
complete medium. Leave the vessel in the sterile field and proceed to thaw the
cryopreserved cells.
4. Place the frozen vial in a 37oC water bath. Hold and rotate the vial gently until the
contents completely thaw. Promptly remove the vial from the water bath, wipe it down
with 70% ethanol, and transfer it to the sterile field.
5. Carefully remove the cap without touching the interior threads. Gently resuspend and
dispense the contents of the vial into the equilibrated, poly-L-lysine-coated culture vessel.
A seeding density of 5,000 cells/cm2
is recommended.
人肠平滑肌细胞Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of residual DMSO in the culture. It is
also important that cells are plated in poly lysine coated culture vessels to promote cell
attachment.
6. Replace the cap or lid of the culture vessel and gently rock the vessel to distribute the
cells evenly. Loosen cap, if necessary, to allow gas exchange.
7. Return the culture vessel to the incubator.
8. For best results, do not disturb the culture for at least 16 hours after the culture has been
initiated. Refresh culture medium the next day to remove residual DMSO and unattached
cells, then every other day thereafter.
Maintaining the culture:
1. Refresh supplemented culture medium the next morning after establishing a culture from
cryopreserved cells.
2. Change the medium every three days thereafter, until the culture is approximately 70%
confluent.
3. Once the culture reaches 70% confluency, change medium every other day until the
culture is approximately 90% confluent.
Subculturing:
1. Subculture when the culture reaches 90% confluency or above.
2. Prepare poly-L-lysine-coated culture vessels (2 μg/cm2
) one day before subculture.
3. Warm complete medium, trypsin/EDTA solution (T/E, Cat. #0103), T/E neutralization
solution (TNS, Cat. #0113), and DPBS (Ca++
- and Mg++
-free, Cat. #0303) to room
temperature. We do not recommend warming reagents and medium in a 37oC water bath
prior to use.
4. Rinse the cells with DPBS.
5. Add 8 ml of DPBS and then 2 ml of T/E solution into flask (in the case of a T-75 flask).
Gently rock the flask to ensure complete coverage of cells by T/E solution. Incubate the
flask in a 37oC incubator for 1 to 2 minutes or until cells completely round up. Use a
microscope to monitor the change in cell morphology.
6. During incubation, prepare a 50 ml conical centrifuge tube with 5 ml of fetal bovine
serum (FBS, Cat. #0500).
7. Transfer T/E solution from the flask to the 50 ml centrifuge tube (a small percent of cells
may detach) and continue to incubate the flask at 37oC for another 1 to 2 minutes (no
solution in the flask at this moment).
8. At the end of incubation, gently tap the side of the flask to dislodge cells from the
surface. Check under a microscope to make sure that all cells detach.
9. Add 5 ml of TNS solution to the flask and transfer detached cells to the 50 ml centrifuge
tube. Rinse the flask with another 5 ml of TNS to collect the residual cells.
10. Examine the flask under a microscope for a successful cell harvest by looking at the
number of cells being left behind; there should be less than 5%.
Note: Use ScienCell T/E solution that is optimized to minimize cell damages due to over
trypsinization.
11. Centrifuge the 50 ml centrifuge tube at 1000 rpm for 5 minutes. Resuspend cells in
culture medium.
12. Count and plate cells in a new poly-L-lysine-coated culture vessel with the recommended
cell density.
Caution: Handling human derived products is potentially biohazardous. Although each cell
strain tests negative for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100%
accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always
wear gloves and safety glasses when working with these materials. Never mouth pipette. We
recommend following the universal procedures for handling products of human origin as the
人肠平滑肌细胞minimum precaution against contamination [1].
[1] Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues.” J Tissue Cult Methods. 11: 191-9. 

ly: Arial">细胞(HEK)提取于人角膜组织,于原代冻存。每管含有细胞数>5×105 cells/ml,此细胞通过Fibronectin免疫荧光染色验证,经测试不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。

推荐培养基:(FM, Cat. No. 2301)

产地:San Diego,US

       

15.视觉细胞系统
    6510 人角膜上皮细胞 (HcorF)( 5×105 )
    6520 人角膜成纤维细胞 (HcorF)( 5×105 )
    6530 人视网膜内皮细胞 (HREC)( 5×105 )
    6540 人视网膜色素上皮细胞(HRPEpiC)(5×105 )
    6550 人晶状体上皮细胞 (HLEpiC)( 5×105 )
    6560 人虹状体上皮细胞 (HIPEpiC)( 5×105 )
    6570 人结膜纤维原细胞(HConF)(5×105 )
    6580 人非色素睫状上皮细胞 (HNPCEpiC)( 5×105 )
    6590 人小梁网细胞(HTMC)(5×105)
  

 

Description

The keratocyte, or corneal fibroblast, is a highly specialized cell that is sandwiched between orthogonally arranged layers of collagen lamellae in the corneal stroma. They play a key role in maintaining the structure and transparency of the cornea as they are the source of stromal collagen and proglycans. They also play important roles in corneal wound healing and tissue repair and are known to undergo phenotypic transformations in wounds due to the influence of growth factors and cytokines [1]. Under normal conditions, the keratocyte in the adult cornea is a relatively quiescent cell. However, in the event of a corneal injury or trauma, the keratocytes differentiate into active, synthesizing cells and rapidly replace damaged stromal matrix. Cultured human keratocytes express functional IL-4Rs [2] and IL-17R [3] on cell surface, suggesting that these cells may contribute to the role of IL-4 and IL-17 as mediators of allergic reactions in the cornea. Changes in gene expression were reproducibly observed on keratocytes after interleukin-1 treatment which provides important insight in gene expression and suggests novel therapeutic targets for the control of corneal inflammation.

HK from ScienCell Research Laboratories are isolated from human cornea. HK are cryopreserved at primary culture. Each vial contains >5 x 10^5 cells in 1 ml volume. HK are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HK are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HK are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.


Recommended Medium

 

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HK in vitro.


Product Use

 

HK are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.


Storage

 

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.


Shipping

 

Dry ice.


Reference

 

[1] Fini, M. E. (1999) Keratocyte and fibroblast phenotype in the repairing cornea. Prog Retin Eye Res 18:529-551.
[2] Fukuda, K., Fujitsu, Y., Kumagai, N., Nishida, T. (2002) Characterization of the interleukin-4 receptor complex in human corneal fibroblasts. Invest Ophthalmol. Vis. Sci. 43(1):183-8.
[3] Maertzdorf, J., Osterhaus, A. D., Verjans, G. M. (2002) IL-17 expression in human herpetic stromal keratitis: modulatory effects on chemokine production by corneal fibroblasts. J. Immunol. 169(10):5897-903.

     
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 Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应 

       近几年的新药研发成功率在逐年下降,其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物,其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本,而且将极大地提高人类的健康质量。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。所以上海中乔新舟生物科技有限公司致力于提供优质原代细胞产品和完善的售后服务。 
     
       美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。

        ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。 确保了实验结果的真实性、重复性和连贯性。

Sciencell公司部分原代细胞目录(如需要其他细胞资料请发邮件至 wwwfudan@163.com索取): 
       
        中乔新舟www.zqxzbio.com专长于为生物医药领域的医疗机构、研究中心、企业、临床医生等提供课题设计、基金联合申请、实验技术服务、论文相关服务、采购外包等整体服务,目前已经成长为国内领先的转化医学外包品牌。
         中乔新舟的PI团队已经发展到专职PI 20人,联席PI 312人,其中大多数拥有海外背景。截止到2009年3月,以PI或联席PI为第一作者发表SCI 论文1682 篇(总IF 值为5721.82,其中影响因子IF>5.0 的论文216 篇,IF>10.0论文32 篇)。
  
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