上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理：

       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。
 

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人心脏成纤维细胞--***心房说明：
货号：6320

心脏成纤维细胞（CF）是构成心脏的主要细胞类型。它们对于心肌细胞起结构支持作用，在生长和病理条件下，负责细胞外基质的合成。CF是心肌损伤反应的重要细胞成分，并能产生旁分泌生长因子。CF的增殖和基质合成对于心肌梗塞、心肌纤维化部位瘢痕形成具有重要作用，并常并发心脏肥厚。CF通过调节整合素的表达来适应病理情况下周围环境的变化。CF的培养已被广泛应用为生理（运动）和病理（高血压）应激下心脏基质重塑研究的模型。     人心脏成纤维细胞(HCF)提取于人心脏组织，于原代冻存。每管含有细胞数>5×105 cells/ml。此细胞通过纤维连接蛋白抗体免疫荧光检测方法验证。经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。 推荐培养基:(FM, Cat. No. 2301) Description   The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction [1], cardiac fibrosis, and is often complicated by cardiac hypertrophy [2]. CF also respond to changing environments in pathophysiological conditions by modulating integrin expression [3]. CF culture has been widely used as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors. HCFaa from ScienCell Research Laboratories are isolated from the human heart. HCF-aa are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCF-aa are characterized by immunofluorescent method with antibody to fibronectin. HCF-aa are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-aa are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories. Recommended Medium   It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HCF-aa in vitro. Product Use   HCF-aa are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage   Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. Shipping   Dry ice. Reference   [1] Sabri, A., Short, J., Guo, J., Steinberg, S. F. (2002) Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes. Circ. Res. 91:532-9. [2] Akiyama-Uchida, Y. et al., (2002) Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts. Hypertension 40:148-54. [3] Burgess, M. L., Terracio, L., Hirozane, T., Borg, T. K. (2002) Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts. Cardiovasc Pathol 11(2):78-87.

       14.心脏细胞系统
    6000 人心脏微血管内皮细胞 (HCMEC)( 5×105 )
    6100 人主动脉内皮细胞 (HAEC)( 5×105 )
    6110 人主动脉平滑肌细胞 (HASMC)( 5×105 )
    6200 人心肌细胞 (HCM) (1×106 )
     6210 ***心肌细胞 (HCMa) (1×106 )
    6300 人心脏成纤维细胞( HCF) ( 5×105 )
    6310 ***心室成纤维细胞 (HCFav)( 5×105 )
    6320 ***心房成纤维细胞 (HCFaa)( 5×105 )