上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理：

       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。
 

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人骨骼肌卫星细胞说明：
货号：3510

4）、如何选用特殊细胞系培养基？  培养某一类型细胞没有固定的培养条件。肺大动脉平滑肌细胞在MEM中培养的细胞，很可能在DMEM或M199中同样很容易生长。总之，首选MEM做粘附细胞培养、RPMI-1640做悬浮细胞培养是一个好的开始。 5）、何时须更换培养基？ 肺大动脉平滑肌细胞 视细胞生长密度而定，或遵照细胞株基本数据上之更换时间，按时更换培养基即可。 6）、可否使用与原先培养条件不同之培养基？  不能。每一细胞株均有其特定使用且已适应之细胞培养基，若骤然使用和原先提供之培养条件不同之培养基，细胞大都无法立即适应，造成细胞无法存活。 >Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37o and return them to culture as quickly as possible with minimal handling! C waterbath Set up of Undifferentiated Expansion of Human Preadipocytes: 4. Primary Human Preadipocytes (HPAs) should be expanded with PAM (cat # 7211) in T- 25 or T-75 flasks, which have been coated with poly-L-lysine and placed for at least 1 hour in the 37°C incubator. 5. Change the medium to fresh supplemented medium the next morning after establishing a culture from cryopreserved cells. For subsequent subcultures, change medium 48 hours after establishing the subculture. 6. Change the medium every other day thereafter, until the culture is ready for subculture. Induction of Adipocyte Differentiation: 8. Plate the preadipocyte suspension in PAM at a density of 10,000 cells/cm2 9. Incubate the cells at 37°C in a 5% CO in the coated flask or plate. 2 Note: Cells should reach 100% confluence before initiating adipocyte induction. humidified incubator for 1-2 days. 10. When the cells are 100% confluent, carefully replace the PAM with Preadipocyte Differentiation Medium (PADM, Cat # 7221). This medium change counts as differentiation day 1. 11. Replace the medium with fresh PADM every 2-3 days. 12. The process of differentiation to mature adipocytes is complete after 5-12 days. Mature adipocytes can be fixed and stained with Oil Red O Solution. Lipid droplets can be observed after 3 days. 13. Mature adipocytes can be maintained in Adipocyte Medium (AdM, Cat. #7201) up to 6 days. Oil Red O Staining Protocol: 0.3g Oil Red O in 100 ml isopropanol .This solution is stable for up to 1 year Stock Oil Red O solution The working solution 1. 3 parts of stock Oil Red O solution and 2 parts of distilled water. (Note: Let it sit at room temperature for 10 min. 2. Filter the working solution completely through the filter funnel. 3. This solution is stable only up to 2 hours. Make it freshly every time you use it. 1. Remove media; rinse cells 2X with PBS. Procedure 2. Fix the cells by covering 10% formaldehyde. 3. Let plates/flasks sit at least for 15 min (or overnight) at room temperature. 4. Make the working solution as described above. 5. Remove fixative solution (10% formaldehyde); gently rinse tissue culture vessels with H2 6. Remove the water; add Oil Red O filtered working solution slowly along the side of culture vessels. Ensure even spreading throughout the wells/flasks. O. 7. Sit > 10 min (1 hour or longer) at room temperature. 8. Rinse with tap water until the water runs clear. 9. View the plates on a phase contrast microscope. Lipids will appear red. Human Preadipocytes-visceral (HPA-v, Cat. # 7210) were observed under a phase contrast microscope. A. The cells were cultivated in Preadipocyte Medium (PAM, Cat # 7211) for 5 days (Control). There were no lipid droplets (10X). B. The cells were cultivated in Preadipocyte Differentiation Medium (PADM, Cat # 7221) for 5 days. Lipid droplets were detected under microscope (20X). 人前脂肪细胞--内脏A B Caution: Handling human derived products is potentially bioharzadous. Although each cell strain tests negative for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100% accurate, ther fore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination [1]. [1]. Grizzle, W. E., and Polt, S. S. (1988) Guidelines to avoid personal contamination by infective agents in research laboratories that use human tissues. J Tissue Culture Methods. 11(4).  font-kerning: 1.0pt; mso-fareast-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-bidi-font-family: Arial">HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。 推荐培养基:(SkMCM, Cat. No. 3501)