少突胶质前体细胞是Raff、Miller 和Noble于1993年首次发现，并已进行了深入的研究。在文献中，前体细胞指少突细胞型星形胶质细胞祖细胞或少突胶质细胞前体细胞。发育中和成熟的中枢神经系统都含有少突胶质前体细胞。少突胶质细胞，中枢神经系统中的髓***脂组成细胞，来源于少突前体细胞。在培养中，少突前体细胞在基本的成纤维细胞生长因子存在的情况下可产生于神经祖细胞或神经干细胞，OPC在血小板衍生的生长因子或星形胶质细胞产生的生长因子存在的情况下才能增殖，并分化成为成熟的少突胶质细胞。正因如此，它们为研究发育转型提供了特殊的细胞种群。 人少突胶质前体细胞－悬浮生长(HOPC-os)提取于人脑组织，经细胞培养纯化后冻存。每管含有细胞数>5×106 cells/ml，此细胞通过A2B5和 Nestin免疫荧光染色验证， 于不同的培养中均能贴壁生长，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。 推荐培养基:It is recommended to use Oligosphere Medium (OsM, Cat. No. 1601) for expanding oligospheres in vitro and use Oligodendrocyte Precursor Cell Differentiation Medium (OPCDM, Cat. No. 1631) for the differentiation of oligospheres. |
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Description The precursor cells for oligodendrocytes were first discovered in 1993 by Raff, Miller and Noble [1] and have been extensively studied. These precursor cells are referred in the literature as either oligodendrocyte-type-2 astrocyte progenitor cells or oligodendrocyte precursor cells (OPC). The developing and adult central nervous system both contain OPC [2, 3]. Oligodendrocytes, the myelin-forming cells of the central nervous system, develop from OPC. In culture, OPC can be generated from neural progenitors or neural stem cells in the presence of basic fibroblast growth factor and they proliferate in presence to platelet-derived growth factor or factors produced by astrocytes [4] and differentiate into mature oligodendrocytes. Because of this, they have provided an exceptional population in which to study developmental transitions.
HOPC-os from ScienCell Research Laboratories are isolated from human oligodendrocyte precursor cell cultures. HOPC-os are cryopreserved and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HOPC-os are characterized by immunofluorescent method with antibodies to A2B5 and nestin. HOPC-os are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOPC-os are guaranteed to further culture at the condition provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Oligodendrocyte Precursor Cell Medium (OPCM, Cat. No. 1601) for expanding HOPC-os in vitro and Oligodendrocyte Precursor Cell Differentiation Medium (OPCDM, Cat. No. 1631) for the differentiation of HOPC-os. Product Use HOPC-os are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. Shipping Dry ice. Reference [1] Raff, M. C., Miller, R. H. and Noble, M. (1983) A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on the culture medium. Nature 303:390-396.
[2] ffrench-Constant, C. and Raff. M. C. (1986) Proliferating bipotential glial progenitor cells in adult rat optic nerve. Nature 319:499-502.
[3] Wolswijk, G. and Noble, M. (1989) Identification of an adult-specific glial progenitor cell. Development 105:387-400.
[4] Noble, M., Murray, K., Stroobant, P., Waterfield, M. D. Riddle, P. (1988) Platelet-derived growth factor promotes division and motility and inhibits premature differentiation of the oligodendrocyte/type-2 astrocyte progenitor cells. Nature 333:560-562. |
