CD1小鼠纹状体神经元
英文名称: MN-s
型号:null    产品货号: XY1560
价格:请致电:010-57128832,18610462672
品牌: 中国/美国
试剂级别: 细胞培养级

CD1小鼠纹状体神经元Cell Specification
The tissue of the central nervous system is made up of two classes of cells that may be broadly
categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain
[1]. Despite great variability in size and shape, all neurons share common morphological
features, the key elements of a highly complex communication network. Neurons are
dynamically polarized cells that serve as the major signaling unit of the nervous system.
MN-s from ScienCell Research Laboratories are isolated from embryonic day 14 mouse
striatum. MN-s are cryopreserved as primary cultures and delivered frozen. Each vial contains
>1 x 106
cells in 1 ml volume. MN-s are characterized by immunofluorescence with antibodies
specific to neurofilament, MAP2, and β-tubulin III. MN-s are negative for mycoplasma, bacteria,
yeast, and fungi. MN-s are guaranteed to further culture under the conditions provided by
ScienCell Research Laboratories; however, MN are not recommended for expanding or long
term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Neuronal Medium (NM, Cat. #1521) for culturing MN-s in vitro.
Product Use
CD1小鼠纹状体神经元MN-s are for research use only. They are not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and
keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
References
[1] Parent A. (1996) “Neurons.” In Carpenters Human Neuroanatomy (9th ed., pp131-198). Quebec: Williams &
Wilkins.
[2] Alberts B, Bray D, Lewis J, Raff M, Roberts M, Watson JD. (1989) Molecular Biology of the Cell (2nd ed.).
New York: Garland.
Instructions for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37oC water bath
and return the cells to culture as quickly as possible with minimal handling!
Initiating the culture:
1. Prepare a poly-L-lysine-coated culture vessel (2 μg/cm2
). It is recommended to use a T-25
flask (alternatively, 3 wells of a 6-well plate or 12 wells of a 24-well plate can be used).
Add 5 ml of sterile water to a T-25 flask and then add 5 μl of poly-L-lysine stock solution
(10 mg/ml, Cat. #0413). Leave the vessel in a 37oC incubator overnight (or for a
minimum of one hour). Rinse the poly-L-lysine-coated vessel twice with sterile water
prior to use.
CD1小鼠纹状体神经元Note: It is important that these cells are plated in poly lysine coated culture vessels to
promote cell attachment.
2. Prepare complete medium. Decontaminate the external surfaces of medium bottle and
medium supplement tubes with 70% ethanol and transfer them to a sterile field.
Aseptically transfer supplement to the basal medium with a pipette. Rinse the supplement
tube with medium to recover the entire volume.
3. Add complete medium to the culture vessel. Leave the vessel in the sterile field and
proceed to thaw the cryopreserved cells.
4. Place the frozen vial in a 37oC water bath. Hold and rotate the vial gently until the
contents completely thaw. Promptly remove the vial from the water bath, wipe it down
with 70% ethanol, and transfer it to the sterile field. Carefully remove the cap without
touching the interior threads.
5. Gently resuspend and dispense the contents of the vial into the poly-L-lysine-coated
culture vessel. A seeding density of 10,000-50,000 cells/cm2
is recommended, with an
optimal range of 20,000-25,000 cells/cm2
.
Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of residual DMSO in the culture.
6. Replace the cap or lid of the culture vessel and gently rock the vessel to distribute the
cells evenly. Loosen cap, if necessary, to allow gas exchange.
7. Return the culture vessel to the incubator.
8. For best results, do not disturb the culture for at least 16 hours after the culture has been
initiated. Refresh culture medium the next day to remove residual DMSO and unattached
cells, then every other day thereafter.
Maintaining the culture:
1. Refresh supplemented culture medium the next morning after establishing a culture from
cryopreserved cells.
CD1小鼠纹状体神经元2. Change the medium every two to three days thereafter.
It is not recommended that neurons be subcultured beyond their initial plating.
Caution: Handling animal derived products is potentially biohazardous. Always wear gloves
and safety glasses when working with these materials. Never mouth pipette. We recommend
following the universal procedures for handling products of human origin as the minimum
precaution against contamination [1].
[1] Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues.” J Tissue Cult Methods. 11: 191-9.

xposed to a dynamic mechnical environment, which influencesomplete coverage of cells by T/E solution. Incubate the
flask in a 37oC incubator for 1 to 2 minutes or until cells completely round up. Use a
microscope to monitor the change in cell morphology.
6. During incubation, prepare a 50 ml conical centrifuge tube with 5 ml of fetal bovine
serum (FBS, Cat. #0500).
7. Transfer T/E solution from the flask to the 50 ml centrifuge tube (a small percent of cells
may detach) and continue to incubate the flask at 37oC for another 1 minute (no solution
in the flask at this moment).
8. At the end of incubation, gently tap the side of the flask to dislodge cells from the
surface. Check under a microscope to make sure that all cells detach.
9. Add 5 ml of TNS solution to the flask and transfer detached cells to the 50 ml centrifuge
tube. Rinse the flask with another 5 ml of TNS to collect the residual cells.
10. Examine the flask under a microscope for a successful cell harvest by looking at the
number of cells being left behind; there should be less than 5%.
Note: Use ScienCell T/E solution that is optimized to minimize cell damages due to over
trypsinization.
11. Centrifuge the 50 ml centrifuge tube at 1000 rpm for 5 minutes. Resuspend cells in
culture medium.
人直肠微血管内皮细胞12. Count and plate cells in a new fibronectin-coated culture vessel with the recommended
cell density.
Caution: Handling human derived products is potentially biohazardous. Although each cell
strain tests negative for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100%
accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always
wear gloves and safety glasses when working with these materials. Never mouth pipette. We
recommend following the universal procedures for handling products of human origin as the
minimum precaution against contamination [1]
[1] Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues.” J Tissue Cult Methods. 11: 191-9.

een fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Westerlund A.,?Hujanen E.,Puistola U.,Turpeenniemi-Hujanen T. (1997) Ovarian fibroblasts stimulate human ovarian cancer cell invasion and expression of 72-kDa gelatinase A (MMP-2) Gynecologic Oncology 67(1)76-82.

 

       

18.卵巢细胞系统
7300 人卵巢微血管内皮细胞(HOMEC) ( 5×105 )
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       近几年的新药研发成功率在逐年下降,其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物,其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本,而且将极大地提高人类的健康质量。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。所以上海中乔新舟生物科技有限公司致力于提供优质原代细胞产品和完善的售后服务。 
     
       美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。

        ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。 确保了实验结果的真实性、重复性和连贯性。

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