大鼠小胶质细胞小胶质细胞是中枢神经系统中一种胶质细胞类型，它是神经胶质细胞网不可或缺的重要组成部分。小胶质细胞在大脑实质从早期向成熟阶段发育的过程中被发现。在大脑发育过程中或中枢神经系统受损或受到病理伤害而发生程序性细胞死亡时，小胶质细胞的作用如同大脑中的巨噬细胞。小胶质细胞被认为是大脑免疫监督的主要细胞，向CD4阳性T细胞表达的II类MHC呈递抗原，具有Fc-介导的巨噬作用并与造血和巨噬细胞组织有许多同样的抗原。另外，有大量证据表明作用，在大脑中小胶质细胞通过与神经元和其它胶质细胞共同作用和产生诸如生长因子、细胞因子和其它因子的方式来参与许多生理和病理过程。

大鼠小胶质细胞(RM)提取于大鼠脑组织，原代冻存，未进行扩增。每管含有细胞数>5×105cells/ml，此细胞通过对OX-42 (CD 11b/c)免疫荧光染色验证，经测试不含有支原体、细菌、酵母和真菌。

Reference
[1] Lee, S. C., Liu, W., Brosnan, C. F. and Dickson, D. W. (1992) Characterization of primary human fetal dissociated central nervous system cultures with an emphasis on microgia. Laboratory Investigation. 67:465-476.
[2] Fedoroff, S., Zhai, R. and Novak, J. P. (1997) Microglia and astroglia have a common progenitor cell. J. Neurosci. Res. 50: 477-486.
[3] Stoll, G. and Jander, S. (1999) The role of microglia and macrophages in the pathophysiology of the CNS. Prog. Neurobiol. 58:233-247. 大鼠小胶质细胞

Description

Microvascular endothelial cells play a central role in inflammation, tumor metastasis, wound healing and tissue engineering [1]. They line the blood vessels and contribute to many biological processes such as hematosis, coagulation and trafficking of lymphocytes. The endothelial cell is of primary importance in the extravasation of leukocytes from the circulation into sites of tissue inflammation. The structure and function of endothelial cells are differentially regulated in space and time. In the case of prostate cancer, the interactions between prostate cancer cells with endothelial cells in the microvessels play an important role in prostate tumor metastasis. Study results suggest that prostatic endothelial cells may be actively involved in prostate growth, due to the secretion of regulatory factors such as IL-6 [2]

HPrMEC from ScienCell Research Laboratories are isolated from human prostate tissue. HPrMEC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPrMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HPrMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPrMEC are guaranteed to further expand for 10 population doublings at the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HPrMEC.

Product Use

HPrMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Richard, L., Velasco, P. and Detmar, M. (1998) Isolation and culture of microvascular endothelial cells. Method in Molecular Medicine 18:261-269.
[2] Stachon, A., Schl?ter, T., K?ller, M., Weisser, H. and Krieg, M. (2001) Primary culture of microvascular endothelila cells from human bening prostatic hyperplasia. Prostate 48(3):156-164.

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Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应

       近几年的新药研发成功率在逐年下降，其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物，其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本，而且将极大地提高人类的健康质量。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。所以上海中乔新舟生物科技有限公司致力于提供优质原代细胞产品和完善的售后服务。

       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。

        ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的真实性、重复性和连贯性。

Sciencell公司部分原代细胞目录（如需要其他细胞资料请发邮件至 wwwfudan@163.com索取）：

        中乔新舟www.zqxzbio.com专长于为生物医药领域的医疗机构、研究中心、企业、临床医生等提供课题设计、基金联合申请、实验技术服务、论文相关服务、采购外包等整体服务，目前已经成长为国内领先的转化医学外包品牌。
         中乔新舟的PI团队已经发展到专职PI 20人，联席PI 312人，其中大多数拥有海外背景。截止到2009年3月，以PI或联席PI为第一作者发表SCI 论文1682 篇（总IF 值为5721.82，其中影响因子IF>5.0 的论文216 篇，IF>10.0论文32 篇）。

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