大鼠腹脊髓神经元
英文名称: Rat Neurons-ventral spinal cord
型号:null    产品货号: XY1570
价格:请致电:010-57128832,18610462672
品牌: 中国/美国
试剂级别: 细胞培养级

大鼠腹脊髓神经元Cell Specification
The tissue of the central nervous system is made up of two classes of cells that may be broadly
categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain
[1]. Despite great variability in size and shape, all neurons share common morphologic features,
which are those of the key elements of a highly complex communication network. The neurons
are the dynamically polarized cells that serve as the major signaling unit of the nervous system.
The human brain is known to contain about 1 x 1011 neurons, each being able to contact at least
10,000 other neurons [2].
RNvsc from ScienCell Research Laboratories were isolated from E14 rat ventral spinal cord.
RNvsc were cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10
cells in 1 ml volume. RNvsc are characterized by immunofluorescent method with antibodies to
neurafilament, MAP2, and beta-tubulin III. RNvsc are negative for mycoplasma, bacteria, yeast
and fungi. RNvsc are guaranteed to further culture in the conditions provided by ScienCell
Research Laboratories.
Recommended Medium
It is recommended to use neuronal medium (NM, Cat. No. 1521) for the culturing of RNvsc in
vitro.
大鼠腹脊髓神经元Product Use
RNvsc are for research use only. They are not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep
the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
Reference
[1] Parent, A. (1996) Neurons in Carpenters Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins,
Quebec, Canada.
[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd.
ed., New York: Garland.
Instruction for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37 C waterbath
and return them to culture as quickly as possible with minimal handling!
Unpacking
1. For crypreserved cells: If there is dry ice in the package and you are not going to culture
cells right way, place cryovial(s) immediately into liquid nitrogen. If there is no dry ice
left in the package, thaw and culture the cells immediately.
2. For proliferating cells: Spray the culture vessel (flask, plate or slide) with 70% ethanol
for disinfection. Transfer the cells into 37 C, 5% CO incubator and allow equilibrating
for 2 hours. After cells have equilibrated, remove shipping medium from the culture
vessel and replace with fresh medium.
Set up culture after receiving the ordering
1. Coat culture vessel with laminin or poly-L-lysine.
Note: It is important that neurons are plated in laminin or poly-L-lysine coated culture
vessels that promote cell attachment and neurites outgrowth (poly-L-lysine coating: coat
flask or plate with poly-L-lysine at 2 μg/ml concentration for one hour and wash the flask
大鼠腹脊髓神经元or plate with sterile water three times).
2. Medium preparation: Decontaminate the external surfaces of medium and medium
supplements with 70% ethanol and transfer them to sterile field. Aseptically open each
supplement tube and add them to the basal medium with a pipette. Rinse each tube with
the medium to recover the entire volume.
3. Set up culture: Prepare one T-45 flask for each cryovial. Add the appropriate amount of
medium to the vessel (recommend for 10 ml/T-45 flask) and allow the flask to equilibrate
in 37 C, 5% CO incubator for at least 30 min.
4. Thawing of cells: Place the vial in a 37 C waterbath, hold and rotate the vial gently until
the contents are completely thawed. Remove the vial from the waterbath immediately,
wipe it dry, and transfer it to a sterile field. Rinse the vial with 70% ethanol, and then
wipe to remove excess. Remove the cap, being careful not to touch the interior threads
with fingers.
5. Using 1 ml eppendorf pipette gently resuspend the cells in the vial and transfer them to
equilibrated culture vessels (a T-45 flask). A high seeding density (>10,000/cm ) is
recommended.
Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of DMSO residue in the culture.
6. Replace the cap or cover, and gently rock the vessel to distribute the cells evenly. Loosen
caps if necessary to permit gas exchange. Return the culture vessels to the incubator.
7. Change the medium 12 hours after plating to remove the residual DMSO and unattached
cells, then every other day thereafter. A health culture will display normal neuron
morphology, and nonvacuole cytoplasm with multiple processes.
大鼠腹脊髓神经元Caution: Handling human derived products is potentially bioharzadous. Although each cell strain testes negative
for HIV, HBV and HCV DNA, proper precautions mush be taken to avoid inadvertent exposure. Always wear gloves
and safety glasses when working these materials. Never mouth pipette. We recommend following the universal
procedures for handling products of human origin as the minimum precaution against contamination [1].
[1]. Grizzle, W. E., and Polt, S. S. (1988) Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues. J Tissue Culture Methods. 11(4).  

.5; mso-char-indent-count: 2.0">骨骼是一类动态的组织,被破骨细胞系统和造骨细胞系统的调节活动不断重塑。造骨细胞是骨骼形成细胞,最初是来源于多能性的间叶干细胞。它们分泌、合成细胞外基质和主要含有I型胶原的的类骨质。类骨质被造骨细胞钙化,在此过程中,细胞在钙化物质中嵌入陷窝中变成骨细胞。当骨骼发育成熟时,造骨细胞的数量和大小减少,但休眠细胞依然可以对伤害做出反应,在骨折愈合过程中产生新的骨骼。在生命的后期,造骨细胞的活动受到甲状旁腺素循环水平的直接影响。造骨细胞表达蛋白酶活性受体1和血管内皮细胞生长因子。研究表明,无论在体内或体外,白血病抑制因子都能与造骨细胞表面结合诱导骨骼形成。颅骨缝合处造骨细胞的补充、增殖、分化和凋亡对颅骨形成起着重要的作用。

    人颅骨成骨细胞(HCO)提取于人颅骨组织,原代冻存。每管含有细胞数>5×105 cells/ml,此细胞通过对AP mineral deposition的细胞化学验证,经测试不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。

推荐培养基:(ObM, Cat. No. 4601)

Description

 

Bone is a dynamic tissue, being continuously remodeled by the coordinated actions of osteoclasts and osteoblast lineage. Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells. They synthesize and secrete organic extracellular matrix, osteoid, which is composed primarily of type I collagen. Osteoid is calcified by osteoblasts and during this process the cells become encased in lacunae within the calcified material and become osteocytes. Osteoblasts express protease-activated receptor-1 and vescular endothelial cell growth factor [1]. Studies show that Leukemia inhibitory factor can bind to the osteoblast cell surface and induce bone formation both in vitro and in vivo [2]. The balance between osteoblast recruitment, proliferation, differentiation and apoptosis in sutures between cranial bones is essential for calvarial bone formation [3].

HCO from ScienCell Research Laboratories are isolated from human calvariae. HCO are cryopreserved at primary cultures and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCO are characterized by the cytochemically detection of AP and mineral deposition. HCO are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCO are guaranteed for 15 population doublings at the conditions provided by ScienCell Research Laboratories.


Recommended Medium

 

It is recommended to use Osteoblast Medium (OsM, Cat. No. 4601) for the culturing of HCO in vitro.


Product Use

 

HCO are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.


Storage

 

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.


Shipping

 

Dry ice.


Reference

 

[1] Steinbrech, D. S., Mehrara, B. J., Saadeh, P. B., Greenwald, J.A., Spector, J. A., Gittes, G. K. and Longaker, M. T. (2000) VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am. J. Physiol. Cell Physiol. 278: C853-C860.
[2] Dazai, S., Akita, S., Hirano, A., Rashid, M. A., Naito, S., Akino, K., Fujii, T. (2000) Leukemia inhibitory factor enhances bone formation in calvarial bone defect. J. Craniofac. Surg. 11(6):513-20.
[3] Marie, P. J., Debiais, F., Hay, E. (2002) Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol. Histopathol.17(3):877-85.

      12.骨骼细胞系统
     4600 人颅骨造骨细胞 (HCO) ( 5×105 )
     4700 人滑膜细胞(HS( 5×105 )
     4800 人髓核细胞(HNPC)(5×105)
 

 

 

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 Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应 

       近几年的新药研发成功率在逐年下降,其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物,其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本,而且将极大地提高人类的健康质量。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。所以上海中乔新舟生物科技有限公司致力于提供优质原代细胞产品和完善的售后服务。 
     
       美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。

        ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。 确保了实验结果的真实性、重复性和连贯性。

Sciencell公司部分原代细胞目录(如需要其他细胞资料请发邮件至 wwwfudan@163.com索取): 
       
        中乔新舟
www.zqxzbio.com专长于为生物医药领域的医疗机构、研究中心、企业、临床医生等提供课题设计、基金联合申请、实验技术服务、论文相关服务、采购外包等整体服务,目前已经成长为国内领先的转化医学外包品牌。
         中乔新舟的PI团队已经发展到专职PI 20人,联席PI 312人,其中大多数拥有海外背景。截止到2009年3月,以PI或联席PI为第一作者发表SCI 论文1682 篇(总IF 值为5721.82,其中影响因子IF>5.0 的论文216 篇,IF>10.0论文32 篇)。
  
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