上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理：

       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。
 

请登录Sciencell公司官方网站（www.zqxzbio.com或www.sciencellonline.com）以确保购买正规公司产品。   

                    
人颅骨成骨细胞说明：
货号：4600

骨骼是一类动态的组织，被破骨细胞系统和造骨细胞系统的调节活动不断重塑。造骨细胞是骨骼形成细胞，最初是来源于多能性的间叶干细胞。它们分泌、合成细胞外基质和主要含有I型胶原的的类骨质。类骨质被造骨细胞钙化，在此过程中，细胞在钙化物质中嵌入陷窝中变成骨细胞。当骨骼发育成熟时，造骨细胞的数量和大小减少，但休眠细胞依然可以对伤害做出反应，在骨折愈合过程中产生新的骨骼。在生命的后期，造骨细胞的活动受到甲状旁腺素循环水平的直接影响。造骨细胞表达蛋白酶活性受体1和血管内皮细胞生长因子。研究表明，无论在体内或体外，白血病抑制因子都能与造骨细胞表面结合诱导骨骼形成。颅骨缝合处造骨细胞的补充、增殖、分化和凋亡对颅骨形成起着重要的作用。     人颅骨成骨细胞(HCO)提取于人颅骨组织，原代冻存。每管含有细胞数>5×105 cells/ml，此细胞通过对AP 和 mineral deposition的细胞化学验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。 推荐培养基:(ObM, Cat. No. 4601) Description   Bone is a dynamic tissue, being continuously remodeled by the coordinated actions of osteoclasts and osteoblast lineage. Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells. They synthesize and secrete organic extracellular matrix, osteoid, which is composed primarily of type I collagen. Osteoid is calcified by osteoblasts and during this process the cells become encased in lacunae within the calcified material and become osteocytes. Osteoblasts express protease-activated receptor-1 and vescular endothelial cell growth factor [1]. Studies show that Leukemia inhibitory factor can bind to the osteoblast cell surface and induce bone formation both in vitro and in vivo [2]. The balance between osteoblast recruitment, proliferation, differentiation and apoptosis in sutures between cranial bones is essential for calvarial bone formation [3]. HCO from ScienCell Research Laboratories are isolated from human calvariae. HCO are cryopreserved at primary cultures and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCO are characterized by the cytochemically detection of AP and mineral deposition. HCO are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCO are guaranteed for 15 population doublings at the conditions provided by ScienCell Research Laboratories. Recommended Medium   It is recommended to use Osteoblast Medium (OsM, Cat. No. 4601) for the culturing of HCO in vitro. Product Use   HCO are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage   Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. Shipping   Dry ice. Reference   [1] Steinbrech, D. S., Mehrara, B. J., Saadeh, P. B., Greenwald, J.A., Spector, J. A., Gittes, G. K. and Longaker, M. T. (2000) VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am. J. Physiol. Cell Physiol. 278: C853-C860. [2] Dazai, S., Akita, S., Hirano, A., Rashid, M. A., Naito, S., Akino, K., Fujii, T. (2000) Leukemia inhibitory factor enhances bone formation in calvarial bone defect. J. Craniofac. Surg. 11(6):513-20. [3] Marie, P. J., Debiais, F., Hay, E. (2002) Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol. Histopathol.17(3):877-85.

      12.骨骼细胞系统
     4600 人颅骨造骨细胞 (HCO) ( 5×105 )
     4700 人滑膜细胞（HS）( 5×105 )
     4800 人髓核细胞(HNPC)(5×105)