上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理: 美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。 ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。 请登录Sciencell公司官方网站(www.zqxzbio.com或www.sciencellonline.com)以确保购买正规公司产品。 人股骨成骨细胞说明: 货号:4610 Description Bone is a dynamic tissue, being continuously remodeled by the coordinated actions of osteoclasts and osteoblast lineage. Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells. They synthesize and secrete organic extracellular matrix, osteoid, which is composed primarily of type I collagen. Osteoid is calcified by osteoblasts and during this process the cells become encased in lacunae within the calcified material and become osteocytes. Osteoblasts express protease-activated receptor-1 and vescular endothelial cell growth factor [1]. Studies show that Leukemia inhibitory factor can bind to the osteoblast cell surface and induce bone formation both in vitro and in vivo [2]. The balance between osteoblast recruitment, proliferation, differentiation and apoptosis in sutures between cranial bones is essential for calvarial bone formation [3]. HO-f from ScienCell Research Laboratories are isolated from human femur. HO-f are cryopreserved at primary cultures and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HO-f are characterized by the cytochemically detection of AP and mineral deposition. HO-f are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HO-f are guaranteed for 15 population doublings at the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Osteoblast Medium (OsM, Cat. No. 4601) for the culturing of HO-f in vitro. Product Use HO-f are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. Shipping Dry ice. Reference [1] Steinbrech, D. S., Mehrara, B. J., Saadeh, P. B., Greenwald, J.A., Spector, J. A., Gittes, G. K. and Longaker, M. T. (2000) VEGF expression in an osteoblast-like cell line is regulated by a hypoxia response mechanism. Am. J. Physiol. Cell Physiol. 278: C853-C860. [2] Dazai, S., Akita, S., Hirano, A., Rashid, M. A., Naito, S., Akino, K., Fujii, T. (2000) Leukemia inhibitory factor enhances bone formation in calvarial bone defect. J. Craniofac. Surg. 11(6):513-20. [3] Marie, P. J., Debiais, F., Hay, E. (2002) Regulation of human cranial osteoblast phenotype by FGF-2, FGFR-2 and BMP-2 signaling. Histol. Histopathol.17(3):877-85. 12.骨骼细胞系统 4600 人颅骨造骨细胞 (HCO) ( 5×105 ) 4700 人滑膜细胞(HS)( 5×105 ) 4800 人髓核细胞(HNPC)(5×105) |