| 上海中乔新舟生物科技有限公司是美国Sciencell公司中国一级代理： 美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。
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人脾成纤维细胞说明：
货号：5530
Description Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [1]. There is good evidence that fibroblasts in different parts of the body are intrinsically different and display different regulatory functions on surrounding cells [2]. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. [3]
HSF from ScienCell Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HSF in vitro. Product Use HSF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. Shipping Dry ice. Reference [1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[2] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[3] Briard, D., Brouty-Boy?, D., Azzarone, B., Jasmin, C. (2002) Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. J Immunol. 168(9):4326-32 |