人视网膜内皮细胞
英文名称: HREC
型号:null    产品货号: 6530
价格:请致电:010-57128832,18610462672
品牌: 美国

上海复蒙基因生物科技有限公司是美国Sciencell公司中国一级代理:

       

       美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发,在全球拥有众多客户。在国内销售8年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。确保了实验结果的重复性和连贯性。
 

请登录Sciencell公司官方网站(www.sciencell-sh.comwww.sciencellonline.com)以确保购买正规公司产品。   

                 
人视网膜内皮细胞说明:
货号:6530

 

 

眼部包含的富含血管和完全无血管的组织,并且紧紧排列的。这种特殊的解剖关系要求对血管的静止和生长平衡有严格的调控。视网膜的血管生成是各种血管细胞,如内皮细胞、外膜细胞、星状胶质细胞,各种正负调节因子产生平衡之间协调的相互作用。在血管生成相关的眼病中,这种微妙的平衡遭到破坏。在大部分致盲性疾病中,血管生成都有重要作用,如糖尿病视网膜病、早产儿视网膜病、老年黄斑退行性变,同时也与许多其它眼病有关。通过研究视网膜内皮细胞,人们越来越清楚了解早期的糖尿病视网膜病和其它的视网膜微血管病。利用人视网膜内皮细胞的培养,可以为我们研究眼血管疾病的病理生理改变提供体外模型。   

    人视网膜内皮细胞(HREC)提取于人视网膜组织,原代冻存。每管含有细胞数>5×105 cells/ml,此细胞通过vWF/Factor VIII CD31 (P-CAM)免疫荧光染色验证,经测试不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌。

推荐培养基:(ECM, Cat. No. 1001)

Description

 

The eye contains highly vascularised and completely avascular tissues in close apposition. This specialized anatomy requires tight regulation of the balance between vascular quiescence and vascular growth. Retinal vascularization is a coordinated interaction of vascular cells, including endothelial cells, pericytes, and astrocytes, and a balanced production of positive and negative regulatory factors [1]. In eye diseases associated with angiogenesis, this delicate balance is disturbed. Angiogenesis plays a crucial role in disorders responsible for most blindness such as diabetic retinopathy, retinopathy of prematurity, age-related macular degeneration, as well as a large number of other eye conditions. Studies of retinal capillary endothelial cells have shed light on the earliest stages of diabetic retinopathy and other diseases of the retinal microvasculature [2, 3]. Human retinal endothelial cell cultures will surely continue to provide an in vitro model in the pathophysiological studies of vascular diseases in the human eye.

HREC from ScienCell Research Laboratories are isolated from human retinal tissue. HREC are cryopreserved on passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HREC are characterized by immunofluorescent method with antibodies to VWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HREC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HREC are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.


Recommended Medium

 

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HREC in vitro.


Product Use

 

HREC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.


Storage

 

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.


Shipping

 

Dry ice.


Reference

 

[1] Su, X., Sorenson, C. M., Sheibani, N. (2003) Isolation and characterization of murine retinal endothelial cells. Molecular Vision 9:171-178.
[2] Su, T., Gillies, M. C. (1992) A simple method for the in vitro culture of human retinal capillary endothelial cells. Invest. Ophthalmol. Vis. Sci. 33:2809-13.
[3] Aguilar, E., Friedlander, M., Gariano, R. F. (2003) Endothelial proliferation in diabetic retinal microaneurysms. Arch. Ophthalmol. 121:740-1.

 
      
 

15.视觉细胞系统
    6510 人角膜上皮细胞 (HcorF)( 5×105 )
    6520 人角膜成纤维细胞 (HcorF)( 5×105 )
    6530 人视网膜内皮细胞 (HREC)( 5×105 )
    6540 人视网膜色素上皮细胞(HRPEpiC)(5×105 )
    6550 人晶状体上皮细胞 (HLEpiC)( 5×105 )
    6560 人虹状体上皮细胞 (HIPEpiC)( 5×105 )
    6570 人结膜纤维原细胞(HConF)(5×105 )
    6580 人非色素睫状上皮细胞 (HNPCEpiC)( 5×105 )
    6590 人小梁网细胞(HTMC)(5×105)
    如需要其他产品资料请发邮件至wwwfudan@163.com索取

        复蒙基因|FMGBio(www.fmgbio.com/   www.sciencell-sh.com/   )成立于2005年, 是专业从事牛血清生产、销售高端的原代细胞类科研产品。这些产品从根本上提高了全球生命医学研究、人类重要疾病药物研发、新药研发的成功率。近几年的新药研发成功率在逐年下降,其根本原因之一就是传统的药物筛选系统是建立在只具有30-40%人类基因群的一系列细胞株上。这样一个有“缺陷型”药物筛选系统所产生的药物用于人体上就会出现许多致命的弱点和不完整性。新一代药物筛选系统是含有一系列近乎完整的人类基因群的原代细胞株。而利用这些原代细胞株所甄别和筛选出来的候选药物,其诊治人类疾病的成功几率将大大增加。这样不但大大节省了新药的开发成本,而且将极大地提高人类的健康质量。

        复蒙基因|FMGBio现有的产品:美国Sciencell(原代细胞、原代细胞专用培养基、无血清培养基)、3D Bioteck(三维细胞培养支架)、年产1000万毫升的内蒙古牛血清生产基地等。
       复蒙基因|FMGBioPI团队已经发展到专职PI 20人,联席PI 312人,其中大多数拥有海外背景。截止到20093月,以PI或联席PI为第一作者发表SCI 论文1682 篇(总IF 值为5721.82,其中影响因子IF>5.0 的论文216 篇,IF>10.0论文32 篇)。

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