Description |  | Monoclonal Antibody against BRST-1 (CU-18) (Formerly Signet Catalog Nos: 610-01, 610-16 L1 Predilute, 610-26 L2 Predilute, 610-36 LV Predilute) |
Intended Use |  | ** In Vitro Diagnostic (IVD) ** |
Clone |  | CU-18 |
Form |  | Tissue Culture Supernatant (in 0.01M PBS + 0.1% NaN3 + 1% BSA) |
Host |  | Mouse |
Species Reactivity |  | Human |
IsoType |  | IgG1 |
Specificity |  | The BRST-1, clone CU-18, monoclonal antibody detects BCA-225, a 225kDa glycoprotein secreted by the T47D breast carcinoma cell line. Strong intracytoplasminc staining is seen in primary and metastatic breast carcinoma cell lines, primary and metastatic breast carcinoma tissue and cervical carcinomas.
This antibody was generated against a protein secreted by the T47D (Clone 11) human breast carcinoma cell line.
Concentrated Format: SIG-3610-1000 [1 mL]
Prediluted Formats: SIG-3610-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems.
SIG-3610-26 [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
SIG-3610-36 [6 mL] is ready-to-use with Biotin based detection systems on Ventana ES and Ventana NexES automated immunostainers. |
Uses |  | This antibody is effective in immunohistochemistry (IHC). |
Suggested Working Dilution |  | The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues Pretreatment: Not required Incubation: 20 minutes at room temperature
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Notes |  | Positive tissue (human): Breast carcinoma |
Storage |  | Store between 2-8°C. |
References |  | Imam SA, et al. Comparison of two microwave based antigen retrieval solutions in unmasking epitopes in formalin-fixed tissue for immunostaining. Anticancer Res 15:1153-8, 1995.
Ansai S, et al. An immunohistochemical study of BCA-225 in various skin cancers. J Dermatol 21(1): 20-4, 1994.
Ma CK, et al. Comparative immunohistochemical study of primary and metastatic carcinomas of the liver. Am J Clin Pathol 99(5):551-7, 1993. |
Warranty/Conditions |  | Covance products may not be resold or modified for resale without prior written approval. |
Rev. Date |  | 3/17/2009 |