Description		Polyclonal Antibody against 6-His (6His, 6xHis, hexaHis)
Form		Antiserum
Host		Rabbit
Specificity		This polyclonal antibody was raised against the peptide HHHHHH (the same epitope as the monoclonal 6-His antibody). This antibody may be preferred over the monoclonal 6-His for experiments in murine systems or in experiments involving two proteins labeled with two different tags. The sequence of the protein flanking the poly His tag does not influence antibody binding.
Uses		This antibody is effective in immunoblotting and immunostaining.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. Western blot:   1:1,000* Immunofluorescence:   1:250
Notes		*Our Posi-Tag Control Protein (PRO-133P) can be used as a helpful positive control for this antibody.
Storage		Store at -20°C. It is suggested that the total volume be divided into useable aliquots upon initial thaw.
References		Janvier K, et. al. HIV-1 Nef Stabilizes the Association of Adaptor Protein Complexes with Membranes. J. Biol. Chem. 278:8725-8732, 2003. Deinert K, Fasiolo F, Hurt EC, Simos G. Arc1p organizes the yeast aminoacyl-tRNA synthetase complex and stabilizes its interaction with the cognate tRNAs. J. Biol. Chem. 276(8):6000-6008, 2001.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		1/1/2004