Description		Beta Amyloid (Aβ) Monoclonal Antibody, 6E10 (Formerly Signet Catalog Nos: 9300-02, 9300-05, 9300-10) Covance is the Exclusive Provider of this Antibody Product
Intended Use		**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use.
Clone		6E10
Form		Ascites
Host		Mouse
Species Reactivity		Human
IsoType		IgG1
Specificity		This antibody is reactive to amino acid residues 1-16 of beta amyloid. The epitope lies within amino acids 3-8 of beta amyloid (EFRHDS). Beta amyloid forms are deposited in the CNS of patients with Alzheimer’s disease and Down’s syndrome. Signet’s beta amyloid antibody 6E10 reacts to the abnormally processed isoforms, as well as precursor forms.
Uses		This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunoaffinity purification, immunoprecipitation, and ELISA.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. IHC: 1:100 - 1:1,000 Tissue Sections: Formalin-fixed human and animal paraffin-embedded brain sections Pretreatment: Formic acid (70%) for 10-30 minutes at room temperature. ELISA: 1:1,000 - 1:10,000
Storage		Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
References		Thakker DR, et al. Intracerebroventricular amyloid-beta antibodies reduce cerebral amyloid angiopathy and associated micro-hemorrhages in aged Tg2576 mice. Proc Natl Acad Sci USA Feb 25, 2009. Kimura N, et al. Age-related changes of intracellular Abeta in cynomolgus monkey brains. Neuropath. Appl. Neurobiol. 31(2):170-80, 2005. Klyubin I, et al. Amyloid beta protein immunotherapy neutralizes Abeta oligomers that disrupt synaptic plasticity in vivo. Nat. Med. 11(5):556-61, 2005. Iijima K, et al. Dissecting pathological effects of human Abeta40 and Abeta42 in Drosophila: a potential model for Alzheimer’s disease. PNAS 101(17):6623-6628, 2004. Li M, et al. Macrophage colony stimulatory factor and interferon-gamma trigger distinct mechanisms for augmentation of beta-amyloid-induced microgliamediated neurotoxicity. J. Neurochem. 91:623-33, 2004. Poduloso JF, et al. Design and chemical synthesis of a magnetic resonance contrast agent with enhanced in vitro binding, high blood-brain barrier permeability, and in vivo targeting to Alzheimer’s disease amyloid plaques. Biochem. 43:6064-6075, 2004. Venezia V, et al. Apoptotic cell death influences the signaling activity of the amyloid precursor protein through SchA and Grb2 adaptor proteins in neuroblastoma SH-SY5Y cells. J. Neurochem. 90:1359-1370, 2004. Kim KS, et al. Production and characterization of monoclonal antibodies reactive to synthetic cerebrovascular amyloid peptide. Neurosci. Res. Comm. 2:121-130, 1988. Kim KS, et al. Neurosci. Res. Comm. 7:113, 1988.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		3/3/2009