Description		Polyclonal Antibody against S100A4
Intended Use		**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use.
Clone		S100A4
Form		Purified Antibody (in 0.1 M NaCl, 0.015 M sodium azide, pH 7.2)
Host		Rabbit
Species Reactivity		Mouse, Rat, Dog, Cow, Cat, Horse
[Ab]		0.72mg/mL
Specificity		The S100A4 protein belongs to the S100 subfamily, which has grown to be one of the largest subfamilies of the EF-hand Ca2+-binding proteins. The S100 proteins are small (10-12 kDa), acidic proteins sharing 25-65% sequence homology. A majority of the S100 genes cluster on human chromosome 1q21. The discovery of this cluster has provided the basis for a new nomenclature for S100 proteins encoded by genes in the 1q21 region. These proteins are now designated S100A1-S100A13. In immunoblotting analysis using recombinant S100 proteins (S100A1, S100A2, S100A4, S100A6 and S100B) only the corresponding antigen (S100A4) was recognized by the A 5114 antibody. No cross-reaction with the other S100 proteins was observed. As demonstrated by immunocytochemistry, the antibody cross-reacts with the S100A4 equivalent protein in cat, cow, dog, horse, mouse and rat. This antibody was raised against purified recombinant human S100A4 monomer expressed by E. coli.
Uses		This antibody is effective in immunoblotting, immunocytochemistry (ICC) and immunohistochemistry (IHC).
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. Western blot: 1:100* IHC: 1:50 - 1:100** Paraffin Sections: The antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heath-induced epitope retrieval is recommended. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure.
Notes		*Predicted MW = 10-12kDa **This antibody has been used at a dilution range of 1:100-1:200 when applied on formalin-fixed, paraffin-embedded sections of human tonsil along with a 20 min heat-induced epitope retrieval, and 30 min primary antibody incubation at room temperature.
Storage		Store between 2-8°C.
References		Schäfer BW, Heizmann CW. The S100 family of EF-hand calcium-binding proteins: functions and pathology. Trends Biochem Sci 21:134-40, 1996. Engelkamp D, Schäfer BW, Mattei MG, Erne P, Heizmann CV. Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E. Proc Natl Acad Sci 90:6547-51, 1993.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		1/2/2008