Monoclonal Antibody against Non-Phosphorylated Neurofilaments H and M
英文名称: Monoclonal Antibody against Non-Phosphorylated Neurofilaments H and M
型号:null    产品货号: SMI-33R
价格:请致电:010-57128832,18610462672
品牌: usa

DescriptionNeurofilament H & M Non-Phosphorylated (SMI 33) Monoclonal Antibody - STERNBERGER MONOCLONALSMonoclonal Antibody against Non-Phosphorylated Neurofilaments H and M
CloneSMI-33
FormAscites Fluid (contains 0.01M sodium azide)
HostMouse
Species ReactivityMammalian, Xenopus
IsoTypeIgM
SpecificityNeurofilament H & M Non-Phosphorylated (SMI 33) Monoclonal Antibody - STERNBERGER MONOCLONALSSMI 33 reacts with a nonphosphorylated epitope accessible in both, phosphorylated and nonphosphorylated neurofilament H and, to a lesser extent, in neurofilament M. In contrast to other antinonphosphoneurofilaments, reaction with isolated normal neurofilaments is not intensified by dephosphorylation. However, in pathologically hyperphosphorylated tissue, reaction with SMI 33 is partially inhibited, and cen be restored by dephosphorylation. Immunocytochemically, SMI 33 visualizes neuronal cell bodies, dendrites and some thick axons in the central and peripheral nervous systems of most mammalian species as well as frog (Xenopus). SMI 33 is more broadly reactive with neuronal cell bodies and processes than SMI 32. There is some binding of SMI 33 to non-neuronal elements in peripheral, non-nervous system tissue, particularly macrophages. In some pathological conditions, neuronal cell body staining with SMI 33 is increased.
UsesNeurofilament H & M Non-Phosphorylated (SMI 33) Monoclonal Antibody - STERNBERGER MONOCLONALSThis antibody is effective in immunoblotting, immunocytochemistry and ELISA.
Suggested Working DilutionNeurofilament H & M Non-Phosphorylated (SMI 33) Monoclonal Antibody - STERNBERGER MONOCLONALSThe optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
  • Western blot: 1:1,000
  • Immunohistochemistry: 1:1,000
  • ELISA: 1:1,000

The extent of permissible dilution of SMI 33 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.
StorageNeurofilament H & M Non-Phosphorylated (SMI 33) Monoclonal Antibody - STERNBERGER MONOCLONALSStore at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Warranty/ConditionsNeurofilament H & M Non-Phosphorylated (SMI 33) Monoclonal Antibody - STERNBERGER MONOCLONALSCovance products may not be resold or modified for resale without prior written approval.
Rev. DateNeurofilament H & M Non-Phosphorylated (SMI 33) Monoclonal Antibody - STERNBERGER MONOCLONALS11/15/2006