Description |  | Monoclonal Antibody against Non-Phosphorylated Neurofilaments H and M | Clone |  | SMI-33 | Form |  | Ascites Fluid (contains 0.01M sodium azide) | Host |  | Mouse | Species Reactivity |  | Mammalian, Xenopus | IsoType |  | IgM | Specificity |  | SMI 33 reacts with a nonphosphorylated epitope accessible in both, phosphorylated and nonphosphorylated neurofilament H and, to a lesser extent, in neurofilament M. In contrast to other antinonphosphoneurofilaments, reaction with isolated normal neurofilaments is not intensified by dephosphorylation. However, in pathologically hyperphosphorylated tissue, reaction with SMI 33 is partially inhibited, and cen be restored by dephosphorylation. Immunocytochemically, SMI 33 visualizes neuronal cell bodies, dendrites and some thick axons in the central and peripheral nervous systems of most mammalian species as well as frog (Xenopus). SMI 33 is more broadly reactive with neuronal cell bodies and processes than SMI 32. There is some binding of SMI 33 to non-neuronal elements in peripheral, non-nervous system tissue, particularly macrophages. In some pathological conditions, neuronal cell body staining with SMI 33 is increased. | Uses |  | This antibody is effective in immunoblotting, immunocytochemistry and ELISA. | Suggested Working Dilution |  | The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000
- Immunohistochemistry: 1:1,000
- ELISA: 1:1,000
The extent of permissible dilution of SMI 33 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined. | Storage |  | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. | Warranty/Conditions |  | Covance products may not be resold or modified for resale without prior written approval. | Rev. Date |  | 11/15/2006 | |