Description		Purified Monoclonal Antibody against CD99 (Ewing's Sarcoma Marker) (Formerly Signet Catalog Nos: 620-01, 620-16 L1 Predilute, 620-26 L2 Predilute, 620-36 LV Predilute)
Intended Use		** In Vitro Diagnostic (IVD) **
Clone		O13
Form		Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA)
Host		Mouse
Species Reactivity		Human
IsoType		IgG1
Specificity		This antibody is specific for p30/32 MIC2, a 30-32 kDa glycoprotein, also referred to as the HBA71 antigen. O13 detects a cell surface antigen expressed in 95% of Ewing's sarcomas (ES). The antigen is also found in some primative neuroectodermal tumors (PNET) and peripheral neuroepitheliomas (PN). Other small round cell tumors (SRCT) that are commonly included in the differential diagnosis of ES, such as neuroblastomas, lymphomas, and rhabdomyosarcomas, as well as other sarcomas, carcinomas, and neuroectodermal tumors are generally negative. In normal tissues, the antigen is strongly expressed in thymocytes and a subset of pancreatic islet cells. This antibody was generated against MeWo human melanoma cells. Concentrated Format: SIG-3620-1000 [1 mL] Prediluted Formats: SIG-3620-16 [6 mL] is ready to use with Peroxidase Anti-Peroxidase detection systems. SIG-3620-26 [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (SIG-32250). SIG-3620-36 [6 mL] is ready-to-use with Biotin based detection systems on Ventana ES, Ventana NexES and Benchmark automated immunostainers.
Uses		This antibody is effective in immunohistochemistry (IHC).
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (SIG-32250) Tissue Sections: Formalin-fixed, paraffin-embedded tissues Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (SIG-31910) Incubation: 20-60 minutes at room temperature
Notes		Positive tissue (human): Ewing’s sarcoma
Storage		Store between 2-8°C.
References		Gu M. Cytokeratin immunoreactivity in Ewing's sarcoma: prevalence in 50 cases confirmed by molecular diagnostic studies. Am J Surg Pathol 24(3):410-416, 2000. Fellinger EJ, et al. Comparison of cell surface antigen HBA71 (p30/32MIC2), neuron-specific enolase, and vimentin in the immunohistochemical analysis of ewing’s sarcoma of bone. Am J Surg Pathol 16(8):746-755, 1992. Fellinger EJ, et al. Biochemical and genetic characterization of the HBA71 Ewing’s sarcoma cell surface antigen. Cancer Res 51:336-340, 1991. Fellinger EJ, et al. Immunohistochemical Analysis of Ewing’s Sarcoma Cell Suface Antigen p30/32MIC2. Am J Surg Pathol 139(2):317-325, 1991. Dracopoli NC, et al. Genes controlling gp25/30 cell surface molecules map to chromosomes X and Y escape X-inactivation. Am J Hum Genet 37:199-207, 1985.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		4/22/2009