Description		Polyclonal Antibody against ProBACE1 (B693)
Intended Use		**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use.
Form		Antiserum
Host		Rabbit
Species Reactivity		Human, Mouse, Rat
Specificity		Processing of the β-amyloid precursor protein (βAPP) by two endopeptidases, β and γ secretases generates the amyloidogenic peptide Aβ, a major factor in the etiology of Alzheimers disease. The beta secretase is a novel membrane bound aspartyl protease which cleaves the β-APP to generate the N-terminus of the Aβ domain. The membrane bound C-terminal fragment generated by the beta secretase cut is subsequently cleaved by the gamma secretase, resulting in the generation and secretion of Aβ. B693 was raised against a peptide sequence CGIRLPLRSGLGGAPLGLRLPR; 22 amino acids of the human proBACE1.
Uses		This antibody is effective in immunoblotting, immunoprecipitation (IP) and immunostaining.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. Western blot: 1:1,000 IP: 1:200 Immunostaining: 1:300
Storage		Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
References		Yan R, Han P, Miao H, Greengard P, Xu H. The transmembrane domain of the Alzheimer's beta-secretase (BACE1) determines its late Golgi localization and access to beta -amyloid precursor protein (APP) substrate. J Biol Chem 276(39):36788-36796, 2001.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		10/6/2008