DNA酶琼脂 英文名称: Dnase Agar | ||||||||||||
型号:null 产品货号: CM0321B | ||||||||||||
价格:请致电:010-57128832,18610462672 | ||||||||||||
品牌: 英国 | ||||||||||||
DNASE AGAR Code: CM0321 For the detection of microbial deoxyribonuclease enzymes, particularly from staphylococci.
* Adjusted as required to meet performance standardsDirections Description Good correlation was shown between DNase production and coagulase activity when testing Staphylococcus aureus strains from clinical samples2,3,4. Both Staphylococcus aureus and Staphylococcus epidermidis produce extracellular DNase5,6,7 but Staphylococcus aureus produces greater quantities1,7. A modification of the medium is to add mannitol (1% w/v) and phenol red or bromothymol blue (0.0025% w/v) as an indicator of mannitol fermentation9. The pH reaction around the colonies must be read before the plate is flooded with acid. The DNase reaction helps in the differentiation and identification of non-pigmented Serratia marcescens8 (positive DNase reaction) from Klebsiella-Enterobacter (negative DNase reaction). Normal HCl is bactericidal and the organisms cannot be recovered from the surface of the agar after flooding. The incorporation of dyes into the medium which can distinguish hydrolysis of DNA is a further modification which avoids the use of acid. Toluidine blue8 and methyl green10 form coloured complexes with polymerised DNA; these colours change as the DNA is hydrolysed. It should be noted that toluidine blue inhibits Gram positive organisms and it is used to detect DNase production by the Enterobacteriaceae. It has been used with ampicillin (30 mg/litre) to demonstrate DNase production by Aeromonas hydrophila from faeces11. Technique |