Description		Polyclonal anti-peptide antibody against GABAA α4 subunit cytosolic loop
Form		Affinity Purified Antibody in 10 mM Hepes (pH 7.5), 150 mM NaCl, 100 ug/mL BSA and 50% glycerol.
Host		Rabbit
Species Reactivity		Rat
[Ab]		~0.15 mg/mL
Specificity		This antibody is specific for GABAA receptor α4 subunit (protein with apparent molecular mass 64 kDA) in rat brain membrane fractions. The antibody was raised against a fusion protein of MBP with amino acid sequence representing the cytosolic loop of the rat A receptor α4 subunit and was purified using chromatography on affinity columns made with the antigen fusion protein.
Uses		This antibody is effective for use in immunoblotting and immunoprecipitation.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. Western blot:   1:1,000* Immunoprecipitation:   8% of all GABAA receptors in dodecylsulfate extracts of rat forebrains (150ul) using 20 ug antibody
Notes		*Predicted MW= 64kD
Storage		Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
References		E Bencsits, V Ebert, V Tretter, W Sieghart. A significant part of native γ-aminobutyric acidA receptors containing α4 subunits do not contain γ or δ subunits. J. Biol. Chem. 274:19613-6, 1999.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		3/3/2004