Description		FITC Labeled Antibody, HA.11
Intended Use		**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use.
Clone		16B12
Form		FITC Labeled Antibody (Stored in PBS + 0.03% Thimerosal + 50% Glycerol)
Host		Mouse
IsoType		IgG1
[Ab]		1 mg/mL
Specificity		Monoclonal antibody HA.11 was raised against the twelve amino acid peptide, CYPYDVPDYASL. This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. HA.11 recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. This antibody was subsequently purified via a protein-G column and conjugated to FITC.
Uses		This antibody is effective in immunofluorescence (IF), immunoblotting, and immunoprecipitation (IP).
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. Western blot: 1:1,000 IF: 1:1,000 IP: 1:150
Notes		This product is also useful in western analysis when combined with our anti-FITC antibody (PRB-163P).
Storage		This product contains 50% glycerol and should be stored at 4°C.
References		Helliwell SB, Losko S, Kaiser CA. Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease. J Cell Biol 153(4):649-662, 2001. Bennett BD, Denis P, Haniu M, Teplow DB, Kahn S, Louis JC, Citron M, Vassar R. A furin-like convertase mediates propeptide cleavage of BACE, the Alzheimer's beta-secretase. J Biol Chem 275(48):37712-37717, 2000. Kolodziej P, Young R. Epitope tagging and protein surveillance. Meth Enzymol 194:508-519, 1991. Field J, Nikawa J, Broek D, MacDonald B, Rodgers K, Wilson I, Lerner R, Wigler M. Purification of RAS responsive adenylyl cyclase complex from Saccharomyces cervisiae by use of an epitope addition method. Mol Cell Biol 8:2159-2165, 1988.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.