英文名称： Monoclonal Antibody against Neuronal Nicotinic Acetylcholine Receptor, β2-Subunits
| Description |  | Monoclonal Antibody against Neuronal Nicotinic Acetylcholine Receptor, β2-Subunits |
| Intended Use | | **Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
| Clone | | 270 |
| Form | | Ascites |
| Host | | Rat |
| Species Reactivity | | Rodent, Chicken |
| IsoType | | IgG2a |
| [Ab] | | 5-6 mg/mL |
| Specificity | | This antibody was generated in rats against purified neuronal nicotinic acetylcholine receptors (AChRs) from chicken brain. It binds to the extracellular surface of neuronal AChR β2-subunits. The antibody binds to native subunits as well as denatured subunits although with lower affinity. Clone 270 is useful for immunoisolating and pharmacologically characterizing AChRs, immunopurification of AChRs and is also useful histologically. The antibody cross-reacts with chicken and rodent AChRs as well as human, albeit with lower affinity. |
| Uses | | This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP). |
| Suggested Working Dilution | | The optimal working dilution should be determined for each specific assay condition.
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| Notes | | *Predicted MW = 57 kDa |
| Storage | | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
| References | | Whiting PJ, Liu R, Morley BJ, Lindstrom JM. Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies. J Neurosci 7:4005-4016, 1987. Whiting PJ, Schoepfer R, Swanson LW, Simmons DM, Lindstrom JM. Functional acetylcholine receptor in PC12 cells reacts with a monoclonal antibody to brain nicotinic receptors. Nature 327:515-518, 1987. Swanson LW, Simmons DM, Whiting PJ, Lindstrom JM. Immunohistochemical localization of neuronal nicotinic receptors in the rodent central nervous system. J Neurosci 7:3334-3342, 1987. Whiting PJ, Lindstrom JM. Pharmacological properties of immuno-isolated neuronal nicotinic receptors. J Neurosci 6:3061-3069, 1986. |
| Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |
| Rev. Date | | 9/13/2007 |