英文名称： Polyclonal Antibody GABAA Receptor α2-Subunit
| Description |  | Polyclonal Antibody GABAA Receptor α2-Subunit |
| Form | | Antiserum |
| Host | | Rabbit |
| Species Reactivity | | Rat. Bovine, human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. |
| Specificity | | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA-Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for α and β subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α subunits of the receptor. Lastly, phosphorylation of β subunits of the receptor has been shown to modulate GABAA-R function. This antibody was developed against a peptide representing the sequence that is specific for the α2 subunit of the receptor. It recognizes the 53kD GABAA-R α2 subunit in Western blots of rat brain membranes. The peptide antigen has no homology with any other GABAA-R subunit. |
| Uses | | This antibody is effective in immunoblotting; other applications not yet determined. |
| Suggested Working Dilution | | The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
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| Notes | | *Predicted MW = 53kD |
| Storage | | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Long term storage should be at -80°C. This shelf-life of this product is approximately 1 year. |
| References | | Kittler JT, McAinsh K, Moss SJ. Mechanisms of GABA A receptor assembly and trafficking - Implications for the modulation of inhibitory neurotransmission. Mol. Neurobiol. 26:2251 - 268, 2002. Whiting PJ, McKernan RM, Wafford KA. Structure and pharmacology of vertebrate, GABAA receptor subtypes. In: International Review of Neurobiology (Bradley RJ, Harris RA, eds.), pp 95, San Diego: Academic Press, 1995. Olsen RW, Tobin AJ. Molecular biology of GABAA receptors. FASEB 4:1469 - 1480, 1990. Pritchett DB, Sontheimer H, Shivers BD, Yner S, Kettenman H, Schofield PR, Seeburg PH. Importance of a novel GABAA receptor subunit for benzodiazepine pharmacology. Nature (London) 338:582-585, 1989. Blair LAC, Levitan ES, Marshall J, Dionne VE, Barnard EA. Single subunits of the GABAA receptor form ion channels with properties of the native receptor. Science 242:577-579, 1988. |
| Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |
| Rev. Date | | 2/1/2007 |