Description		Polyclonal Antibody against DOPA Decarboxylase
Form		Affinity Purified Antibody (in10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA and 50% glycerol).
Host		Rabbit
Species Reactivity		Rat
Specificity		Aromatic L-amino acid decarboxylase (AADC; DOPA decarboxylase; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin. It is also involved in the biosynthesis of trace amines. The immunogen for this antibody was SDS denatured, recombinant bovine aromatic DDC expressed in E. coli and purified from inclusion bodies. The antiserum was subsequently purified on an affinity column bound with recombinant bovine DDC.
Uses		This antibody is effective in immunoblotting.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. Western blot: 1:1,000* Dot blot: 1:1,000
Notes		*Predicted MW=55kD
Storage		Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. For long-term storage, keep antibody at -80°C.
References		Haycock JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, Kish SJ. Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J. Neurochem. 87:574 - 585, 2003. Waymire JC, Haycock JW Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J. Neurochem. 81:589 - 593, 2002. Berry MD, Juorio AV, Li XM, Boulton AA. Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem. Res. 21:1075 -1087, 1996.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		11/8/2006