Description |  | Polyclonal Antibody against Glial Fibrillary Acidic Protein |
Form |  | Affinity Purified Antibody |
Host |  | Chicken |
Species Reactivity |  | Human, Rat, Mouse, Cat |
IsoType |  | IgY |
[Ab] |  | 20 mg/mL |
Specificity |  | Glial fibrillary acidic protein (GFAP) is the 10nm or intermediate filament protein found specifically in astrocytic cells in the central nervous system. Antibodies to GFAP are therefore widely used to identify astrocytic cells in situ and in tissue culture. This antibody was developed using bovine spinal cord GFAP purified from a Triton X-100 extract of myelin associated material and further purified by centrifugation and ion exchange chromatography in 6m urea on DEAE cellulose.
This antibody was raised agianst GFAP in chickens. |
Uses |  | This antibody is effective in immunofluorescence. |
Suggested Working Dilution |  | The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000
- Immunofluorescence: 1:1,000
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Storage |  | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. |
Warranty/Conditions |  | Covance products may not be resold or modified for resale without prior written approval. |
Rev. Date |  | 11/8/2006 |