Description		Chicken Polyclonal Antibody against Neurofilament NF-L
Form		Purified Antibody
Host		Chicken
IsoType		IgY
[Ab]		30 mg/mL
Specificity		Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, α-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant and are biochemically stable. This antibody was generated against bovine spinal cord intermediate filaments. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, chickens were boosted with recombinant mouse NF-L purified from bacteria.
Uses		This antibody is effective in immunoblotting and immunofluorescence.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. Western blot: 1:10,000 Immunofluorescence: 1:5,000
Storage		Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		3/2/2005