Description		Monoclonal Antibody against Synaptobrevin
Clone		SP10
Form		Ascites
Host		Mouse
Species Reactivity		Human, Rodent, Pig
IsoType		IgM
[Ab]		3-5 mg/mL
Specificity		This antibody was raised against a crude synaptic preparation of post mortem human brain. SP10 reacts with native, denatured and recombinant synaptic vesicle proteins synaptobrevin/VAMP-1 (aa 1-118) and VAMP-2 expressed in a bacterial system (aa 33-96). The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections.
Uses		This antibody is effective in immunoblotting, ELISA and immunocytochemistry.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. Western blot: 1:1,000* Immunocytochemistry: 1:1,000 ELISA: 1:5,000
Notes		*Predicted MW = 16 kD
Storage		Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
References		Honer WG, Hu L, Davies P. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res. 609:9-20, 1993.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.
Rev. Date		1/1/2004